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Journal article
Stanniocalcin 2 (STC2) expression promotes post-radiation survival, migration and invasion of nasopharyngeal carcinoma cells
Cancer management and research, v 11, pp 6411-6424
11 Jul 2019
PMID: 31372045
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background:
Stanniocalcin 2 (STC2) expression is upregulated under multiple stress conditions including hypoxia, nutrient starvation and radiation. Overexpression of STC2 correlates with tumor progression and poor prognosis.
Purpose:
We previously demonstrated that overexpression of STC2 in nasopharyngeal carcinomas (NPC) positively correlates with radiation resistance and tumor metastasis, two major clinical obstacles to the improvement of NPC management. However, it remains elusive whether STC2 expression is a critical contributing factor for post-radiation survival and metastasis of NPC cells.
Materials and methods:
Using the radiation resistant CNE2 cell line as a model, we examined the importance of STC2 expression for post-radiation survival, migration and invasion. Here, we report the establishment of STC2 knockout lines (CNE2-STC2-KO) using the CRISPR/Cas9-based genome editing technique.
Results:
Compared with the parental line, STC2-KO cells showed similar proliferation and morphology in normal culture conditions, and loss of STC2 did not compromise the cell tumorigenicity in nude mice model. However, STC2-KO lines demonstrated increased sensitivity to X-radiation under either normoxic or hypoxic conditions. Particularly, upon X-radiation, parental CNE2 cells only slightly whereas STC2-KO cells remarkably decreased the migration and invasion ability. Cell cycle analysis revealed that loss of STC2 accumulated cells in G
1
and G
2
/M phases but decreased S-population.
Conclusion:
These data indicate that the expression of STC2, which can be stimulated by metabolic or therapeutic stresses, is one important factor to promote survival and metastasis of post-radiation NPC cells. Therefore, targeting STC2 or relative downstream pathways may provide novel strategies to overcome radiation resistance and metastasis of NPC.
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Details
- Title
- Stanniocalcin 2 (STC2) expression promotes post-radiation survival, migration and invasion of nasopharyngeal carcinoma cells
- Creators
- Huocong He - Fujian Provincial Cancer HospitalShuo Qie - Drexel UniversityQiaojuan Guo - Fujian Provincial Cancer HospitalShuyang Chen - Department of Biology, Drexel University, College of Arts and Sciences, Philadelphia, PA 19104, USA.Changyan Zou - Fujian Provincial Cancer HospitalTianzhu Lu - Department of Radiation Oncology, Fujian Cancer Hospital & Fujian Medical University, Fujian Provincial Key Laboratory of Translational Cancer MedicineYing Su - Fujian Provincial Cancer HospitalJingfeng Zong - Fujian Provincial Cancer HospitalHanchuan Xu - Fujian Provincial Cancer HospitalDan He - Department of Biology, Drexel University, College of Arts and Sciences, Philadelphia, PA 19104, USA.Yun Xu - Fujian Provincial Cancer HospitalBijuan Chen - Fujian Provincial Cancer HospitalJianji Pan - Fujian Provincial Cancer HospitalNianli Sang - Drexel UniversityShaojun Lin - Department of Radiation Oncology, Fujian Cancer Hospital & Fujian Medical University, Fujian Provincial Key Laboratory of Translational Cancer Medicine
- Publication Details
- Cancer management and research, v 11, pp 6411-6424
- Publisher
- Dove
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Biology
- Web of Science ID
- WOS:000475300400001
- Scopus ID
- 2-s2.0-85073397424
- Other Identifier
- 991019167749504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Oncology