Journal article
Starch based polyhydroxybutyrate production in engineered Escherichia coli
Bioprocess and biosystems engineering, v 38(8), pp 1479-1484
01 Aug 2015
PMID: 25820819
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Every year, the amount of chemosynthetic plastic accumulating in the environment is increasing, and significant time is required for decomposition. Bio-based, biodegradable plastic is a promising alternative, but its production is not yet a cost effective process. Decreasing the production cost of polyhydroxyalkanoate by utilizing renewable carbon sources for biosynthesis is an important aspect of commercializing this biodegradable polymer. An Escherichia coli strain that expresses a functional amylase and accumulate polyhydroxybutyrate (PHB), was constructed using different plasmids containing the amylase gene of Panibacillus sp. and PHB synthesis genes from Ralstonia eutropha. This engineered strain can utilize starch as the sole carbon source. The maximum PHB production (1.24 g/L) was obtained with 2 % (w/v) starch in M9 media containing 0.15 % (w/v) yeast extract and 10 mM glycine betaine. The engineered E. coli SKB99 strain can accumulate intracellular PHB up to 57.4 % of cell dry mass.
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Details
- Title
- Starch based polyhydroxybutyrate production in engineered Escherichia coli
- Creators
- Shashi Kant Bhatia - Konkuk UniversityYoung-Ha Shim - Konkuk UniversityJong-Min Jeon - Konkuk UniversityChristopher J. Brigham - University of Massachusetts DartmouthYong-Hyun Kim - Konkuk UniversityHyun-Joong Kim - Konkuk UniversityHyung-Min Seo - Konkuk UniversityJu-Hee Lee - Konkuk UniversityJung-Ho Kim - Konkuk UniversityDa-Hye Yi - Konkuk UniversityYoo Kyung Lee - Korea Polar Research InstituteYung-Hun Yang - Konkuk University
- Publication Details
- Bioprocess and biosystems engineering, v 38(8), pp 1479-1484
- Publisher
- Springer Nature
- Number of pages
- 6
- Grant note
- NRF-2013R1A1A2A10004690 / National Research Foundation of Korea (NRF) - Ministry of Education; National Research Foundation of Korea; Ministry of Education (MOE), Republic of Korea PE14030 / Korea Polar Research Institute; Korea Polar Research Institute of Marine Research Placement (KOPRI) 20133030000300 / Energy Efficiency and Resources of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) - Korea Government Ministry of Trade, Industry and Energy 201-101-007; 405-112-0382 / Korea Ministry of Environment; Ministry of Environment (ME), Republic of Korea KU Brain Pool of Konkuk University
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000358934700006
- Scopus ID
- 2-s2.0-84961290890
- Other Identifier
- 991021229896704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Engineering, Chemical