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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of cell–selectin interaction kinetics
Biomaterials, v 33(20), pp 5004-5012
01 Jul 2012
PMID: 22494889
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Dynamic cell–microenvironment interactions regulate many biological events and play a critical role in tissue regeneration. Cell homing to targeted tissues requires well balanced interactions between cells and adhesion molecules on blood vessel walls. However, many stem cells lack affinity with adhesion molecules. It is challenging and clinically important to engineer these stem cells to modulate their dynamic interactions with blood vessels. In this study, a new chemical strategy was developed to engineer cell–microenvironment interactions. This method allowed the conjugation of peptides onto stem cell membranes without affecting cell viability, proliferation or multipotency. Mesenchymal stem cells (MSCs) engineered in this manner showed controlled firm adhesion and rolling on E-selectin under physiological shear stresses. For the first time, these biomechanical responses were achieved by tuning the binding kinetics of the peptide-selectin interaction. Rolling of engineered MSCs on E-selectin is mediated by a Ca2+ independent interaction, a mechanism that differs from the Ca2+ dependent physiological process. This further illustrates the ability of this approach to manipulate cell–microenvironment interactions, in particular for the application of delivering cells to targeted tissues. It also provides a new platform to engineer cells with multiple functionalities.
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Details
- Title
- Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of cell–selectin interaction kinetics
- Creators
- Hao Cheng - Massachusetts Institute of TechnologyMarta Byrska-Bishop - Koch Institute for Integrative Cancer Research At MITCathy T. Zhang - Massachusetts Institute of TechnologyChristian J. Kastrup - University of British ColumbiaNathaniel S. Hwang - Seoul National UniversityAlbert K. Tai - Tufts UniversityWon Woo Lee - Seoul National University Bundang HospitalXiaoyang Xu - Koch Institute for Integrative Cancer Research At MITMatthias Nahrendorf - Harvard Medical SchoolRobert Langer - Massachusetts Institute of TechnologyDaniel G. Anderson - Massachusetts Institute of Technology
- Publication Details
- Biomaterials, v 33(20), pp 5004-5012
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering
- Web of Science ID
- WOS:000304502900004
- Scopus ID
- 2-s2.0-84860361008
- Other Identifier
- 991021886800304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials