Journal article
Stem cell mobilization by hyperbaric oxygen
American journal of physiology. Heart and circulatory physiology, v 290(4), pp H1378-1386
Apr 2006
PMID: 16299259
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We hypothesized that exposure to hyperbaric oxygen (HBO(2)) would mobilize stem/progenitor cells from the bone marrow by a nitric oxide (*NO) -dependent mechanism. The population of CD34(+) cells in the peripheral circulation of humans doubled in response to a single exposure to 2.0 atmospheres absolute (ATA) O(2) for 2 h. Over a course of 20 treatments, circulating CD34(+) cells increased eightfold, although the overall circulating white cell count was not significantly increased. The number of colony-forming cells (CFCs) increased from 16 +/- 2 to 26 +/- 3 CFCs/100,000 monocytes plated. Elevations in CFCs were entirely due to the CD34(+) subpopulation, but increased cell growth only occurred in samples obtained immediately posttreatment. A high proportion of progeny cells express receptors for vascular endothelial growth factor-2 and for stromal-derived growth factor. In mice, HBO(2) increased circulating stem cell factor by 50%, increased the number of circulating cells expressing stem cell antigen-1 and CD34 by 3.4-fold, and doubled the number of CFCs. Bone marrow *NO concentration increased by 1,008 +/- 255 nM in association with HBO(2). Stem cell mobilization did not occur in knockout mice lacking genes for endothelial *NO synthase. Moreover, pretreatment of wild-type mice with a *NO synthase inhibitor prevented the HBO(2)-induced elevation in stem cell factor and circulating stem cells. We conclude that HBO(2) mobilizes stem/progenitor cells by stimulating *NO synthesis.
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Details
- Title
- Stem cell mobilization by hyperbaric oxygen
- Creators
- Stephen R Thom - University of PennsylvaniaVeena M Bhopale - University of PennsylvaniaOmaida C Velazquez - University of PennsylvaniaLee J Goldstein - University of PennsylvaniaLynne H Thom - University of PennsylvaniaDonald G Buerk - University of Pennsylvania
- Publication Details
- American journal of physiology. Heart and circulatory physiology, v 290(4), pp H1378-1386
- Publisher
- American Physiological Society (APS)
- Grant note
- AT-00428 / NCCIH NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000235972500008
- Scopus ID
- 2-s2.0-33646119423
- Other Identifier
- 991019231628904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cardiac & Cardiovascular Systems
- Peripheral Vascular Disease
- Physiology