Journal article
Steroid affected cytokines in aspirin-exacerbated respiratory disease
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY, v 12(10), p1232
Oct 2022
PMID: 35032094
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background Patients with aspirin-exacerbated respiratory disease (AERD) are among the most challenging rhinologic patients to treat. AERD has a complex inflammatory milieu of lipid mediators and cytokines. In this study we evaluated cytokine differences in the complex AERD environment at the mucus, epithelial, and tissue levels. Methods Samples were acquired at the time of sinus surgery from 21 patients (seven steroid-treated, 14 untreated) with aspirin challenge-confirmed AERD. Three methods (sponge adsorption, epithelial brushing, tissue biopsy) were used to acquire samples from the respective sinus sampling sites (mucus, polyp epithelium, and full-thickness polyp) of each patient. We measured and compared 16 cytokine concentrations in AERD patients with or without prednisone treatment using the Luminex platform. Results In most sampling sites, IL-5, IL-6, IL-10, IL-13, IL-33, CCL20, and TNF-alpha were detected at higher concentrations than IFN-gamma, IL-1 beta, IL-17A, IL-4, IL-22, IL-17E/IL25, and GM-CSF. Each sampling site had a different pattern of cytokine levels, and except for IL-5 and IL-25 there was no correlation among sampling methods for each cytokine tested. The most notable and significant decreases in cytokines from those treated with prednisone were observed in the epithelium for IL-5, IL-10, IL-33, and IFN-gamma. Conclusions In the epithelial samples, type 2-associated cytokines IL-5 and IL-33, the anti-inflammatory cytokine IL-10, and IFN-gamma were lower in AERD patients treated with prednisone. This work serves as a basis to assess therapeutic-induced mucosal cytokine responses in AERD and indicates that the site of cytokine measurement is an important consideration when assessing results.
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Details
- Title
- Steroid affected cytokines in aspirin-exacerbated respiratory disease
- Publication Details
- INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY, v 12(10), p1232
- Publisher
- WILEY; HOBOKEN
- Grant note
- This work was supported by ARS Friends in Research Award (MAK), NHLBI K08 HL151911 (MAK), VA BX005432 (NAC), VA CX001617 (NAC).
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Drexel University
- Web of Science ID
- WOS:000745848300001
- Scopus ID
- 2-s2.0-85123477426
- Other Identifier
- 991021861297304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Otorhinolaryngology