Journal article
Stimulation of Retinal Capillary Pericyte Protein and Collagen Synthesis in Culture by High-Glucose Concentration
Diabetes (New York, N.Y.), v 33(8), pp 785-789
01 Aug 1984
PMID: 6745504
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The influence of glucose concentration on cell multiplication and protein synthesis was studied in synchronized, long-term cultures of bovine retinal microvessel pericytes. The cell multiplication rate and the mitotic rate were reduced in media containing 20 mM glucose to 57% and 54%, respectively, of that obtained in media containing 5 mM glucose. Elevated glucose, however, did not change the DNA content of individual cells.
Protein and collagen synthesis were measured by the incorporation of radioactive proline and lysine, or the posttranslational production of hydroxyproline and hydroxylysine, respectively. High glucose stimulated protein and collagen synthesis per cell 2.2 ± 0.10 (SD) and 2.1 ± 0.06 times, respectively. Aspirin (0.5 mM), an inhibitor of nonenzymatic glycosylation, did not alter the effect of elevated glucose concentration on protein and collagen synthesis.
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Details
- Title
- Stimulation of Retinal Capillary Pericyte Protein and Collagen Synthesis in Culture by High-Glucose Concentration
- Creators
- Weiye Li - Department of Ophthalmology, Scheie Eye Institute, School of Medicine, University of Pennsylvania Philadelphia, PennsylvaniaShuyu Shen - Department of Ophthalmology, Scheie Eye Institute, School of Medicine, University of Pennsylvania Philadelphia, PennsylvaniaMahin Khatami - Department of Ophthalmology, Scheie Eye Institute, School of Medicine, University of Pennsylvania Philadelphia, PennsylvaniaJohn H Rockey - Department of Ophthalmology, Scheie Eye Institute, School of Medicine, University of Pennsylvania Philadelphia, Pennsylvania
- Publication Details
- Diabetes (New York, N.Y.), v 33(8), pp 785-789
- Publisher
- American Diabetes Association
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Ophthalmology [Historical]
- Web of Science ID
- WOS:A1984TB55400013
- Scopus ID
- 2-s2.0-0021150336
- Other Identifier
- 991019299006004721
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- Web of Science research areas
- Endocrinology & Metabolism