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Journal article
Stimulatory Effects of Insulin-like Growth Factor-I on Growth Plate Chondrogenesis Are Mediated by Nuclear Factor-κB p65
The Journal of biological chemistry, v 283(49), pp 34037-34044
05 Dec 2008
PMID: 18922796
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Insulin-like growth factor-I (IGF-I) is an important regulator of
endochondral ossification. However, little is known about the signaling
pathways activated by IGF-I in growth plate chondrocytes. We have previously
shown that NF-κB-p65 facilitates growth plate chondrogenesis. In this
study, we first cultured rat metatarsal bones with IGF-I and/or pyrrolidine
dithiocarbamate (PDTC), a known NF-κB inhibitor. The IGF-I-mediated
stimulation of metatarsal growth and growth plate chondrogenesis was
neutralized by PDTC. In rat growth plate chondrocytes, IGF-I induced
NF-κB-p65 nuclear translocation. The inhibition of NF-κB-p65
expression and activity (by p65 short interfering RNA and PDTC, respectively)
in chondrocytes reversed the IGF-I-mediated induction of cell proliferation
and differentiation and the IGF-I-mediated prevention of cell apoptosis.
Moreover, the inhibition of the phosphatidylinositol 3-kinase and Akt
abolished the effects of IGF-I on NF-κB activation. In conclusion, our
findings indicate that IGF-I stimulates growth plate chondrogenesis by
activating NF-κB-p65 in chondrocytes.
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Details
- Title
- Stimulatory Effects of Insulin-like Growth Factor-I on Growth Plate Chondrogenesis Are Mediated by Nuclear Factor-κB p65
- Creators
- Shufang Wu - St. Christopher's Hospital for ChildrenDoris Fadoju - St. Christopher's Hospital for ChildrenGeoffrey Rezvani - St. Christopher's Hospital for ChildrenFrancesco De Luca - St. Christopher's Hospital for Children
- Publication Details
- The Journal of biological chemistry, v 283(49), pp 34037-34044
- Publisher
- American Society for Biochemistry and Molecular Biology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine
- Web of Science ID
- WOS:000261277700031
- Scopus ID
- 2-s2.0-57749101280
- Other Identifier
- 991019168198104721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology