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Journal article
Stochastic dynamic study of optical transition properties of single GFP-like molecules
Journal of biological physics, v 42(2), pp 271-297
Mar 2016
PMID: 26841730
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Due to high fluctuations and quantum uncertainty, the processes of single-molecules should be treated by stochastic methods. To study fluorescence time series and their statistical properties, we have applied two stochastic methods, one of which is an analytic method to study the off-time distributions of certain fluorescence transitions and the other is Gillespie's method of stochastic simulations. These methods have been applied to study the optical transition properties of two single-molecule systems, GFPmut2 and a Dronpa-like molecule, to yield results in approximate agreement with experimental observations on these systems. Rigorous oscillatory time series of GFPmut2 before it unfolds in the presence of denaturants have not been obtained based on the stochastic method used, but, on the other hand, the stochastic treatment puts constraints on the conditions under which such oscillatory behavior is possible. Furthermore, a sensitivity analysis is carried out on GFPmut2 to assess the effects of transition rates on the observables, such as fluorescence intensities.
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Details
- Title
- Stochastic dynamic study of optical transition properties of single GFP-like molecules
- Creators
- Hanbing Lin - Drexel UniversityJian-Min Yuan - Drexel University
- Publication Details
- Journal of biological physics, v 42(2), pp 271-297
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Physics
- Web of Science ID
- WOS:000372251200007
- Scopus ID
- 2-s2.0-84961137584
- Other Identifier
- 991019169794604721
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- Web of Science research areas
- Biophysics