Journal article
Strain-specific virulence phenotypes of Streptococcus pneumoniae assessed using the Chinchilla laniger model of otitis media
PloS one, v 3(4), pp e1969-e1969
09 Apr 2008
PMID: 18398481
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Abstract
Streptococcus pneumoniae [Sp] infection is associated with local and systemic disease. Our current understanding of the differential contributions of genetic strain variation, serotype, and host response to disease phenotype is incomplete. Using the chinchilla model of otitis media [OM] we investigated the disease phenotype generated by the laboratory strain TIGR4 and each of thirteen clinical strains (BS68-75, BS290, BS291, BS293, BS436 and BS437); eleven of the thirteen strains have been genomically sequenced.
For each strain 100 colony forming units were injected bilaterally into the tympanic bullae of 6 young adult chinchillas under general anesthesia. All animals were examined daily for local and systemic disease by a blinded observer. Pneumatic otoscopy was used to evaluate local disease, and behavioral assessments served as the measure of systemic disease. Virulence scoring was performed using a 4-point scale to assess four clinical parameters [severity and rapidity of local disease onset; and severity and rapidity of systemic disease onset] during a 10-day evaluation period. Highly significant variation was observed among the strains in their ability to cause disease and moribundity.
As expected, there was a significant correlation between the rapidity of systemic disease onset and severity of systemic disease; however, there was little correlation between the severity of otoscopic changes and severity of systemic disease. Importantly, it was observed that different strains of the same serotype produced as broad an array of disease phenotypes as did strains of different serotypes. We attribute these phenotypic differences among the strains to the high degree of genomic plasticity that we have previously documented.
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Details
- Title
- Strain-specific virulence phenotypes of Streptococcus pneumoniae assessed using the Chinchilla laniger model of otitis media
- Creators
- Michael L Forbes - Department of Pediatrics, Allegheny General Hospital, Pittsburgh, Pennsylvania, United States of AmericaEdward HorseyN Luisa HillerFarrel J BuchinskyJay D HayesJames M ComplimentTodd HillmanSuzanne EzzoKai ShenRandy KeefeKaren BarbadoraJ Christopher PostFen Ze HuGarth D Ehrlich
- Publication Details
- PloS one, v 3(4), pp e1969-e1969
- Publisher
- Public LIbrary of Science (PLOS); United States
- Grant note
- DC04173 / NIDCD NIH HHS DC02148 / NIDCD NIH HHS DC05659 / NIDCD NIH HHS R01 DC004173 / NIDCD NIH HHS R01 DC005659 / NIDCD NIH HHS R01 DC002148 / NIDCD NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Otolaryngology (and Head and Neck Surgery)
- Web of Science ID
- WOS:000260795500034
- Scopus ID
- 2-s2.0-44849108763
- Other Identifier
- 991014878088604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology