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Journal article
Strategies for neurotrophin-3 and chondroitinase ABC release from freeze-cast chitosan-alginate nerve-guidance scaffolds
Journal of tissue engineering and regenerative medicine, v 11(1), pp 285-294
01 Jan 2017
PMID: 24889394
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Freeze casting, or controlled unidirectional solidification, can be used to fabricate chitosan-alginate (C-A) scaffolds with highly aligned porosity that are suitable for use as nerve-guidance channels. To augment the guidance of growth across a spinal cord injury lesion, these scaffolds are now evaluated in vitro to assess their ability to release neurotrophin-3 (NT-3) and chondroitinase ABC (chABC) in a controlled manner. Protein-loaded microcapsules were incorporated into C-A scaffolds prior to freeze casting without affecting the original scaffold architecture. In vitro protein release was not significantly different when comparing protein loaded directly into the scaffolds with release from scaffolds containing incorporated microcapsules. NT-3 was released from the C-A scaffolds for 8weeks in vitro, while chABC was released for up to 7weeks. Low total percentages of protein released from the scaffolds over this time period were attributed to limitation of diffusion by the interpenetrating polymer network matrix of the scaffold walls. NT-3 and chABC released from the scaffolds retained bioactivity, as determined by a neurite outgrowth assay, and the promotion of neurite growth across an inhibitory barrier of chondroitin sulphate proteoglycans. This demonstrates the potential of these multifunctional scaffolds for enhancing axonal regeneration through growth-inhibiting glial scars via the sustained release of chABC and NT-3. Copyright (c) 2014 John Wiley & Sons, Ltd.
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Details
- Title
- Strategies for neurotrophin-3 and chondroitinase ABC release from freeze-cast chitosan-alginate nerve-guidance scaffolds
- Creators
- Nicola L. Francis - Drexel UniversityPhilipp M. Hunger - Drexel UniversityAmalie E. Donius - Drexel UniversityUlrike G. K. Wegst - Dartmouth CollegeMargaret A. Wheatley - Drexel University
- Publication Details
- Journal of tissue engineering and regenerative medicine, v 11(1), pp 285-294
- Publisher
- Wiley
- Number of pages
- 10
- Grant note
- 1R01DE015633 / National Institutes of Health (NIH)-NIDCR; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) Bryon Riesch Paralysis Foundation 0654313 / NSF-IGERT Grant R01DE015633 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) NSF-DMI-0521309 / National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000394173600026
- Scopus ID
- 2-s2.0-84901737663
- Other Identifier
- 991019169786404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Cell & Tissue Engineering
- Cell Biology
- Engineering, Biomedical