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Journal article
Structural Basis of Arp2/3 Complex Inhibition by GMF, Coronin, and Arpin
Journal of molecular biology, v 429(2)
20 Jan 2017
PMID: 27939292
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Abstract
The evolutionarily conserved Arp2/3 complex plays a central role in nucleating the branched actin filament arrays that drive cell migration, endocytosis, and other processes. To better understand Arp2/3 complex regulation, we used single-particle electron microscopy to compare the structures of Arp2/3 complex bound to three different inhibitory ligands: glia maturation factor (GMF), Coronin, and Arpin. Although the three inhibitors have distinct binding sites on Arp2/3 complex, they each induced an "open" nucleation-inactive conformation. Coronin promoted a standard (previously described) open conformation of Arp2/3 complex, with the N-terminal beta-propeller domain of Coronin positioned near the p35/ARPC2 subunit of Arp2/3 complex. GMF induced two distinct open conformations of Arp2/3 complex, which correlated with the two suggested binding sites for GMF. Furthermore, GMF synergized with Coronin in inhibiting actin nucleation by Arp2/3 complex. Arpin, which uses VCA-related acidic (A) motifs to interact with the Arp2/3 complex, induced the standard open conformation, and two new masses appeared at positions near Arp2 and Arp3. Furthermore, Arpin showed additive inhibitory effects on Arp2/3 complex with Coronin and GMF. Together, these data suggest that Arp2/3 complex conformation is highly polymorphic and that its activities can be controlled combinatorially by different inhibitory ligands. (C) 2016 The Authors. Published by Elsevier Ltd.
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Details
- Title
- Structural Basis of Arp2/3 Complex Inhibition by GMF, Coronin, and Arpin
- Creators
- Olga S. Sokolova - Lomonosov Moscow State UniversityAngelina Chemeris - École PolytechniqueSiyang Guo - Brandeis UniversitySalvatore L. Alioto - Brandeis UniversityMeghal Gandhi - Brandeis UniversityShae Padrick - Drexel UniversityEvgeniya Pechnikova - A.V. Shubnikov Institute of CrystallographyViolaine David - École PolytechniqueAlexis Gautreau - École PolytechniqueBruce L. Goode - Brandeis University
- Publication Details
- Journal of molecular biology, v 429(2)
- Publisher
- Elsevier
- Number of pages
- 12
- Grant note
- HHMI; Howard Hughes Medical Institute 17-14-00043 / Russian Science Foundation; Russian Science Foundation (RSF) 14-14-00234 / Russian Science Foundation; Russian Science Foundation (RSF) I-1544 / Welch Foundation; The Welch Foundation R01GM056322 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R01-GM56322; GM063691 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000393542200005
- Scopus ID
- 2-s2.0-85008230376
- Other Identifier
- 991019167984804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology