Journal article
Structural and functional integration of human forebrain organoids with the injured adult rat visual system
Cell stem cell, v 30(2), pp 137-152
02 Feb 2023
PMID: 36736289
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Brain organoids created from human pluripotent stem cells represent a promising approach for brain repair. They acquire many structural features of the brain and raise the possibility of patient-matched repair. Whether these entities can integrate with host brain networks in the context of the injured adult mammalian brain is not well established. Here, we provide structural and functional evidence that human brain organoids successfully integrate with the adult rat visual system after transplantation into large injury cavities in the visual cortex. Virus-based trans-synaptic tracing reveals a polysynaptic pathway between organoid neurons and the host retina and reciprocal connectivity between the graft and other regions of the visual system. Visual stimulation of host animals elicits responses in organoid neurons, including orientation selectivity. These results demonstrate the ability of human brain organoids to adopt sophisticated function after insertion into large injury cavities, suggesting a translational strategy to restore function after cortical damage.
[Display omitted]
•Human brain organoids integrate with the injured visual cortex of adult rats•Organoid grafts are synaptically connected to the host retina and visual system•Organoid neurons respond to host visual stimulation and adopt feature selectivity
Chen and colleagues demonstrate that human brain organoids can integrate structurally and functionally with the injured adult mammalian brain. Organoid grafts connect synaptically with the rat brain and adopt the function of the visual cortex. These findings support brain organoid transplantation as a therapeutic strategy for restoring cortical function.
Metrics
Details
- Title
- Structural and functional integration of human forebrain organoids with the injured adult rat visual system
- Creators
- Dennis Jgamadze - Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USAJames T. Lim - Brigham and Women's HospitalZhijian Zhang - University of PennsylvaniaPaul M. Harary - University of PennsylvaniaJames Germi - University of PennsylvaniaKobina Mensah-Brown - University of PennsylvaniaChristopher D. Adam - University of PennsylvaniaEhsan Mirzakhalili - University of PennsylvaniaShikha Singh - University of PennsylvaniaJiahe Ben Gu - University of PennsylvaniaRachel Blue - University of PennsylvaniaMehek Dedhia - University of PennsylvaniaMarissa Fu - Drexel University College of Medicine, Philadelphia, PA 19129, USAFadi Jacob - University of PennsylvaniaXuyu Qian - University of PennsylvaniaKimberly Gagnon - University of PennsylvaniaMatthew Sergison - Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USAOceane Fruchet - University of PennsylvaniaImon Rahaman - University of PennsylvaniaHuadong Wang - Shenzhen Institutes of Advanced TechnologyFuqiang Xu - University of PennsylvaniaRui Xiao - University of PennsylvaniaDiego Contreras - University of PennsylvaniaJohn A. Wolf - Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USAHongjun Song - Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USAGuo-li Ming - University of PennsylvaniaHan-Chiao Isaac Chen - Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
- Publication Details
- Cell stem cell, v 30(2), pp 137-152
- Publisher
- Elsevier
- Number of pages
- 16
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine
- Web of Science ID
- WOS:000934257900001
- Scopus ID
- 2-s2.0-85147107501
- Other Identifier
- 991021861199804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell & Tissue Engineering
- Cell Biology