Journal article
Structural and functional studies of CCAAT/enhancer binding sites within the human immunodeficiency virus type 1 subtype C LTR
Biomedicine & pharmacotherapy, v 64(10), pp 672-680
2010
PMID: 20970301
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human immunodeficiency virus type 1 (HIV-1) subtype C, which is most predominant in sub-Saharan Africa as well as in Asia and India, is the most prevalent subtype worldwide. A large number of transcription factor families have been shown to be involved in regulating HIV-1 gene expression in T lymphocytes and cells of the monocyte-macrophage lineage. Among these, proteins of the CCAAT/enhancer binding protein (C/EBP) family are of particular importance in regulating HIV-1 gene expression within cells of the monocytic lineage during the course of hematologic development and cellular activation. Few studies have examined the role of C/EBPs in long terminal repeat (LTR)-directed viral gene expression of HIV-1 subtypes other than subtype B. Within subtype B viruses, two functional C/EBP sites located upstream of the TATA box are required for efficient viral replication in cells of the monocyte-macrophage lineage. We report the identification of three putative subtype C C/EBP sites, upstream site 1 and 2 (C-US1 and C-US2) and downstream site 1 (C-DS1). C-US1 and C-DS1 were shown to form specific DNA-protein complexes with members of the C/EBP family (C/EBPα, β, and δ). Functionally, within the U-937 monocytic cell line, subtype B and C LTRs were shown to be equally responsive to C/EBPβ-2, although the basal activity of subtype C LTRs appeared to be higher. Furthermore, the synergistic interaction between C/EBPβ-2 and Tat with the subtype C LTR was also observed in U-937 cells as previously demonstrated with the subtype B LTR.
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- Title
- Structural and functional studies of CCAAT/enhancer binding sites within the human immunodeficiency virus type 1 subtype C LTR
- Creators
- Yujie Liu - Department of Microbiology and Immunology, Center for Molecular Virology and Translational Neuroscience, Center for Molecular Therapeutics and Resistance, Center for International Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900, Queen Lane, Philadelphia, Pennsylvania 19129, USAMichael R Nonnemacher - Department of Microbiology and Immunology, Center for Molecular Virology and Translational Neuroscience, Center for Molecular Therapeutics and Resistance, Center for International Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900, Queen Lane, Philadelphia, Pennsylvania 19129, USADevin L Stauff - Department of Microbiology and Immunology, Center for Molecular Virology and Translational Neuroscience, Center for Molecular Therapeutics and Resistance, Center for International Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900, Queen Lane, Philadelphia, Pennsylvania 19129, USALuna Li - Department of Microbiology and Immunology, Center for Molecular Virology and Translational Neuroscience, Center for Molecular Therapeutics and Resistance, Center for International Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900, Queen Lane, Philadelphia, Pennsylvania 19129, USAAnupam Banerjee - Department of Microbiology and Immunology, Center for Molecular Virology and Translational Neuroscience, Center for Molecular Therapeutics and Resistance, Center for International Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900, Queen Lane, Philadelphia, Pennsylvania 19129, USABryan Irish - Department of Microbiology and Immunology, Center for Molecular Virology and Translational Neuroscience, Center for Molecular Therapeutics and Resistance, Center for International Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900, Queen Lane, Philadelphia, Pennsylvania 19129, USAEvelyn Kilareski - Department of Microbiology and Immunology, Center for Molecular Virology and Translational Neuroscience, Center for Molecular Therapeutics and Resistance, Center for International Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900, Queen Lane, Philadelphia, Pennsylvania 19129, USANirmala Rajagopalan - Freedom Foundation, 180, Henur Cross, Bangalore, 560043 IndiaJoyce B Suchitra - Freedom Foundation, 180, Henur Cross, Bangalore, 560043 IndiaZafar K Khan - Department of Microbiology and Immunology, Center for Molecular Virology and Translational Neuroscience, Center for Molecular Therapeutics and Resistance, Center for International Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900, Queen Lane, Philadelphia, Pennsylvania 19129, USAUdaykumar Ranga - Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, 560064 IndiaBrian Wigdahl - Department of Microbiology and Immunology, Center for Molecular Virology and Translational Neuroscience, Center for Molecular Therapeutics and Resistance, Center for International Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900, Queen Lane, Philadelphia, Pennsylvania 19129, USA
- Publication Details
- Biomedicine & pharmacotherapy, v 64(10), pp 672-680
- Publisher
- Elsevier SAS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000286087600005
- Scopus ID
- 2-s2.0-78649864889
- Other Identifier
- 991014878155004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Medicine, Research & Experimental
- Pharmacology & Pharmacy