Journal article
Structure of a Filament-Like Actin Trimer Bound to the Bacterial Effector VopL
Cell, v 155(2), pp 423-434
10 Oct 2013
PMID: 24120140
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Bacterial pathogens use secreted effector proteins to subvert host-cell defenses. VopL is an effector protein from
Vibrio parahaemolyticus
that nucleates actin filaments. VopL consists of a VopL C-terminal Domain (VCD) and a tandem array of three WASP homology 2 (WH2) motifs. Here we report the crystal structure of the VCD dimer bound to actin. The VCD binds three actin monomers in a spatial arrangement close to that in the canonical actin filament. In this configuration each actin can readily accommodate a WH2 motif. The data suggest a mechanism of nucleation wherein VopL creates filament-like structures, organized by the VCD and delivered by the WH2 array, that can template addition of new monomers. Similarities with Arp2/3 complex and formin proteins suggest that organization of monomers into filament-like structures is a general and central feature of actin nucleation.
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Details
- Title
- Structure of a Filament-Like Actin Trimer Bound to the Bacterial Effector VopL
- Creators
- Jacob A. Zahm - The University of Texas Southwestern Medical CenterShae B. Padrick - The University of Texas Southwestern Medical CenterZhucheng Chen - The University of Texas Southwestern Medical CenterChi W. Pak - The University of Texas Southwestern Medical CenterAli A. Yunus - The University of Texas Southwestern Medical CenterLisa Henry - The University of Texas Southwestern Medical CenterDiana R. Tomchick - The University of Texas Southwestern Medical CenterZhe Chen - The University of Texas Southwestern Medical CenterMichael K. Rosen - Howard Hughes Medical Institute
- Publication Details
- Cell, v 155(2), pp 423-434
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000325719800018
- Scopus ID
- 2-s2.0-84897584115
- Other Identifier
- 991020836338604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology