Journal article
Studies of the cation binding properties of an oligomeric derivative of prostaglandin B 1
Prostaglandins, leukotrienes and essential fatty acids, v 37(1), pp 37-43
1989
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Abstract
The ionophoretic activity of PGB
x, an oligomeric mixture synthesized from 15-dehydro PGB
1, with different cations was measured using arsenazo III-entrapped liposomes. The order of ionophoretic activity was Zn
2+ > Co
2+ > Mn
2+ > Cu
2+ > Ca
2+ > Ba
2+ > Sr
2+ > Mg
2+. The intrinsic fluorescence of PGB
x was quenched by the binding of divalent cations as well as by La
3+ and H
+. Quenching by K
+ and Na
+ was minimal. The order of quenching strength of divalent cations was Zn
2+ > Co
2+ > Cu
2+ = Mn
2+ > Ca
2+ > Ba
2+ > Sr
2+ > Mg
2+. Binding affinities of these cations determined by a murexide indicator method were in good agreement with that determined by the fluorescence quenching reaction. The cation binding affinity of PGB
x in aqueous solutions correlates with the ionophoretic activity in liposomes. The binding affinity for K
+ was estimated from the inhibition by K
+ of Ca
2+ binding by PGB
x. Although PGB
x has a lower selectivity for divalent cation binding than the ionophore A23187, the characteristics of the binding affinity of these two compounds for various ions were similar. The pK of PGB
x as determined by fluorescence quenching was 6.7. The molecular weight of the divalent cation binding unit was estimated to be about 680, with each PGB
x molecule having three such binding sites. The binding of Ca
2+ to such a site is one-to-one.
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Details
- Title
- Studies of the cation binding properties of an oligomeric derivative of prostaglandin B 1
- Creators
- T. KometaniT.M. DevlinS.Tsuyoshi Ohnishi
- Publication Details
- Prostaglandins, leukotrienes and essential fatty acids, v 37(1), pp 37-43
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1989AF80300006
- Scopus ID
- 2-s2.0-0024318542
- Other Identifier
- 991019184289404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology
- Endocrinology & Metabolism