Journal article
Studies on the development and behavior of the dystrophic growth cone, the hallmark of regeneration failure, in an in vitro model of the glial scar and after spinal cord injury
The Journal of neuroscience, v 24(29), pp 6531-6539
21 Jul 2004
PMID: 15269264
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have developed a novel in vitro model of the glial scar that mimics the gradient of proteoglycan found in vivo after spinal cord injury. In this model, regenerated axons from adult sensory neurons that extended deeply into the gradient developed bulbous, vacuolated endings that looked remarkably similar to dystrophic endings formed in vivo. We demonstrate that despite their highly abnormal appearance and stalled forward progress, dystrophic endings are extremely dynamic both in vitro and in vivo after spinal cord injury. Time-lapse movies demonstrated that dystrophic endings continually send out membrane veils and endocytose large membrane vesicles at the leading edge, which were then retrogradely transported to the rear of the "growth cone." This direction of movement is contrary to membrane dynamics that occur during normal neurite outgrowth. As further evidence of this motility, dystrophic endings endocytosed large amounts of dextran both in vitro and in vivo. We now have an in vitro model of the glial scar that may serve as a potent tool for developing and screening potential treatments to help promote regeneration past the lesion in vivo.
Metrics
Details
- Title
- Studies on the development and behavior of the dystrophic growth cone, the hallmark of regeneration failure, in an in vitro model of the glial scar and after spinal cord injury
- Creators
- Veronica J Tom - Department of Neurosciences, Case Western Reserve University, Cleveland, Ohio 44106, USAMichael P SteinmetzJared H MillerCatherine M DollerJerry Silver
- Publication Details
- The Journal of neuroscience, v 24(29), pp 6531-6539
- Publisher
- Society for Neuroscience; United States
- Grant note
- R01 NS025713 / NINDS NIH HHS R37 NS025713 / NINDS NIH HHS NS 25713 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000222821800014
- Scopus ID
- 2-s2.0-3242759236
- Other Identifier
- 991014878619804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences