Journal article
Subpopulations of Bone Marrow Mesenchymal Stem Cells Exhibit Differential Effects in Delaying Retinal Degeneration
Current molecular medicine, v 16(6), pp 567-581
01 Jan 2016
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Bone marrow mesenchymal stem cells (BMSCs) have a therapeutic role in retinal degeneration (RD). However, heterogeneity of BMSCs may be associated with differential therapeutic effects in RD. In order to confirm this hypothesis, two subsets of rat BMSCs, termed rBMSC1 and rBMSC2, were obtained, characterized and functionally evaluated in the treatment of RD of Royal College of Surgeons (RCS) rats. Both subpopulations expressed mesenchymal stem cells (MSC) markers CD29 and CD90, but were negative for hemacyte antigen CD11b and CD45 expression. In comparison with rBMSC2, rBMSC1 showed higher rate of proliferation, stronger colony formation, and increased adipogenic potential, whereas rBMSC2 exhibited higher osteogenic potential. Microarray analysis showed differential gene expression patterns between rBMSC1 and rBMSC2, including functions related to proliferation, differentiation, immunoregulation, stem cell maintenance and division, survival and antiapoptosis. After subretinal transplantation in RCS rats, rBMSC1 showed stronger rescue effect than rBMSC2, including increased b-wave amplitude, restored retinal nuclear layer thickness, and decreased number of apoptotic photoreceptors, whereas the rescue function of rBMSC2 was essentially not better than the control. Histological analysis also demonstrated that rBMSC1 possessed a higher survival rate than rBMSC2 in subretinal space. In addition, treatment of basic fibroblast growth factor, an accompanying event in subretinal injection, triggered more robust increase in secretion of growth factors by rBMSC1 as compared to rBMSC2. Taken together, these results have suggested that the different therapeutic functions of BMSC subpopulations are attributed to their distinct survival capabilities and paracrine functions. The underlying mechanisms responsible for the different functions of BMSC subpopulation may lead to a new strategy for the treatment of RD.
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Details
- Title
- Subpopulations of Bone Marrow Mesenchymal Stem Cells Exhibit Differential Effects in Delaying Retinal Degeneration
- Creators
- P Li - Tongji UniversityH Tian - Tongji UniversityZ Li - Tongji UniversityL Wang - Tongji UniversityF Gao - Tongji UniversityQ Ou - Tongji UniversityC Lian - Tongji UniversityW Li - Drexel University, Ophthalmology [Historical]C Jin - Tongji UniversityJ Zhang - Tongji UniversityJ-Y Xu - Tongji UniversityJ Wang - Tongji UniversityJ Zhang - Tongji UniversityF Wang - Tongji UniversityL Lu - Tongji UniversityG-T Xu - Tongji University
- Publication Details
- Current molecular medicine, v 16(6), pp 567-581
- Publisher
- Bentham
- Number of pages
- 15
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Ophthalmology [Historical]
- Web of Science ID
- WOS:000382640200006
- Scopus ID
- 2-s2.0-84981504654
- Other Identifier
- 991019168566704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Medicine, Research & Experimental