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Superoxide anion production and phagocytosis of crystals by cultured endothelial cells
Journal article   Open access

Superoxide anion production and phagocytosis of crystals by cultured endothelial cells

Gerald F. Falasca, Anand Ramachandrula, Kathleen A. Kelley, Carolyn R O'Connor and Antonio J. Reginato
Arthritis and rheumatism, v 36(1), pp 105-116
Jan 1993
DOI: https://doi.org/10.1002/art.1780360118
PMID: 8381009
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.1002/art.1780360118View
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Abstract

Abstract Objective. To show that cultured human umbilical vein endothelial cells (HUVEC) are capable of phagocytizing inflammation‐causing crystals and of generating superoxide anion (SOA) during phagocytosis. Methods. The superoxide dismutase‐inhibitable reduction of nitroblue tetrazolium (NBT) dye was used as a measure of SOA production. Phagocytosis was quantified by light microscopy and confirmed by transmission electron microscopy. Cytochrome C was also studied but was found to undergo spontaneous reduction by monosodium urate (MSU) without cells. Results. Crystals of MSU, calcium oxalate, hydroxyapatite, and calcium pyrophosphate dihydrate (CPPD) were phagocytized and, except for the CPPD crystals, induced NBT reduction. Cholesterol and cholesterol monohydrate were neither phagocytized nor did they induce NBT reduction. Conclusions. Endothelial cells may be a significant source of oxygen radicals in crystal‐associated and other arthritides.

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Rheumatology
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