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Journal article
Supramolecular arrangement of protein in nanoparticle structures predicts nanoparticle tropism for neutrophils in acute lung inflammation
Nature nanotechnology, v 17(1), pp 86-97
Jan 2022
PMID: 34795440
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This study shows that the supramolecular arrangement of proteins in nanoparticle structures predicts nanoparticle accumulation in neutrophils in acute lung inflammation (ALI). We observed homing to inflamed lungs for a variety of nanoparticles with agglutinated protein (NAPs), defined by arrangement of protein in or on the nanoparticles via hydrophobic interactions, crosslinking and electrostatic interactions. Nanoparticles with symmetric protein arrangement (for example, viral capsids) had no selectivity for inflamed lungs. Flow cytometry and immunohistochemistry showed NAPs have tropism for pulmonary neutrophils. Protein-conjugated liposomes were engineered to recapitulate NAP tropism for pulmonary neutrophils. NAP uptake in neutrophils was shown to depend on complement opsonization. We demonstrate diagnostic imaging of ALI with NAPs; show NAP tropism for inflamed human donor lungs; and show that NAPs can remediate pulmonary oedema in ALI. This work demonstrates that structure-dependent tropism for neutrophils drives NAPs to inflamed lungs and shows NAPs can detect and treat ALI.
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Details
- Title
- Supramolecular arrangement of protein in nanoparticle structures predicts nanoparticle tropism for neutrophils in acute lung inflammation
- Creators
- Jacob W Myerson - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaPriyal N Patel - University of PennsylvaniaKathryn M Rubey - University of PennsylvaniaMarco E Zamora - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaMichael H Zaleski - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaNahal Habibi - University of MichiganLandis R Walsh - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaYi-Wei Lee - University of Massachusetts AmherstDavid C Luther - University of Massachusetts AmherstLaura T Ferguson - University of PennsylvaniaOscar A Marcos-Contreras - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaPatrick M Glassman - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaLiudmila L Mazaleuskaya - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaIan Johnston - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaElizabeth D Hood - University of PennsylvaniaTea Shuvaeva - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaJichuan Wu - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaHong-Ying Zhang - University of PennsylvaniaJason V Gregory - University of MichiganRaisa Y Kiseleva - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaJia Nong - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaTilo Grosser - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaColin F Greineder - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaSamir Mitragotri - Harvard UniversityGeorge S Worthen - University of PennsylvaniaVincent M Rotello - University of Massachusetts AmherstJoerg Lahann - University of MichiganVladimir R Muzykantov - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaJacob S Brenner - University of Pennsylvania
- Publication Details
- Nature nanotechnology, v 17(1), pp 86-97
- Publisher
- Springer Nature
- Number of pages
- 12
- Grant note
- R01 HL157189 / NHLBI NIH HHS T32 HL007915 / NHLBI NIH HHS K08 HL130430 / NHLBI NIH HHS UL1 TR001878 / NCATS NIH HHS R01 HL160694 / NHLBI NIH HHS R01 HL125462 / NHLBI NIH HHS K08 HL138269 / NHLBI NIH HHS R01 EB022641 / NIBIB NIH HHS F32 HL151026 / NHLBI NIH HHS F31 HL154662 / NHLBI NIH HHS R01 HL153510 / NHLBI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000720230500001
- Scopus ID
- 2-s2.0-85119440125
- Other Identifier
- 991020100201704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology