Journal article
Surface-enhanced Raman spectroscopy as a tool for detecting Ca2+ mobilizing second messengers in cell extracts
Analytical chemistry (Washington), v 82(16), pp 6770-6774
15 Aug 2010
PMID: 20704365
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Understanding of calcium signaling pathways in cells is essential for elucidating the mechanisms of both normal cell function and cancer development. Calcium messengers play the crucial role for intracellular Ca(2+) release. We propose a new approach to detecting the calcium second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) in cell extracts using surface-enhanced Raman spectroscopy (SERS). Currently available radioreceptor binding and enzymatic assays require extensive sample preparation and take more than 12 h. With a SERS sensor, NAADP can be detected in less than 1 min without any special sample preparation. To the best of our knowledge, this is the first demonstration of using SERS for calcium signaling applications.
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Details
- Title
- Surface-enhanced Raman spectroscopy as a tool for detecting Ca2+ mobilizing second messengers in cell extracts
- Creators
- Elina A Vitol - Department of Electrical and Computer Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USAEugen BrailoiuZulfiya OrynbayevaNae J DunGary FriedmanYury Gogotsi
- Publication Details
- Analytical chemistry (Washington), v 82(16), pp 6770-6774
- Publisher
- American Chemical Society; Washington, DC
- Grant note
- R01 HL090804 / NHLBI NIH HHS HL 90804 / NHLBI NIH HHS HL 90804-01A2S1 / NHLBI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering; Materials Science and Engineering; Surgery
- Web of Science ID
- WOS:000280758400005
- Scopus ID
- 2-s2.0-77955627940
- Other Identifier
- 991014877775704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical