Journal article
Survival of Escherichia coli O157:H7 in various soil particles: importance of the attached bacterial phenotype
Biology and fertility of soils, v 53(2), 209
01 Feb 2017
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The risk of enteropathogens to food and water is highly dependent on their survival in soil environments. Here, the effects of soil type, particle size, the presence of natural organic matter (NOM) or Fe/Al (hydro)oxides on pathogenic Escherichia coli O157:H7 survival in sterilized soil particles were assessed through survival, attachment, metabolic activity, and qRT-PCR analyses. The abundance of inoculated E. coli O157:H7 in Brown soil (Alfisol) particles increased 0.6-1.4 log(10) CFU/g within 3 days (except for NOM-stripped clay), while that in Red soil (Ultisol) particles decreased rapidly in 8 days post-inoculation. Additionally, survival of bacteria was significantly enhanced when Fe/Al (hydro)oxides had been removed from Red soil particles. For the two soils, E. coli O157:H7 survived the longest in NOM-present clays and the bacterial adenosine 5'-triphosphate (ATP) levels were 0.7-2.0 times greater in clays than in sands and silts on day 8. Moreover, clays were more effective than silts and sands in binding cells and changing the expressions of acetate pathway-associated genes (pta and ackA). For silts and sands, E. coli O157:H7 decayed more rapidly in the presence of NOM and similar trends of bacterial ATP levels were observed between NOM-stripped and NOM-present soil particles, indicating that the primary role of NOM was not as a nutrient supply. These findings indicate that soil particles function mainly through attachment to change the metabolic pathway of E. coli O157:H7 and ultimately impact the survival of bacterial pathogens in soils.
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Details
- Title
- Survival of Escherichia coli O157:H7 in various soil particles: importance of the attached bacterial phenotype
- Creators
- Xing Liu - Huazhong Agricultural UniversityChunhui Gao - Huazhong Agricultural UniversityDandan Ji - Huazhong Agricultural UniversitySharon L. Walker - University of California SystemQiaoyun Huang - Huazhong Agricultural UniversityPeng Cai - Huazhong Agricultural University
- Publication Details
- Biology and fertility of soils, v 53(2), 209
- Publisher
- Springer Nature
- Number of pages
- 11
- Grant note
- 41522106 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 2016YFD0800206 / National Key Research Program of China 2015CB150504 / National Basic Research Program of China
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:000392372200006
- Scopus ID
- 2-s2.0-85007247664
- Other Identifier
- 991021229881104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Soil Science