Journal article
Swi1 Prevents Replication Fork Collapse and Controls Checkpoint Kinase Cds1
Molecular and cellular biology, v 23(21), pp 7861-7874
Nov 2003
PMID: 14560029
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The replication checkpoint is a dedicated sensor-response system activated by impeded replication forks. It stabilizes stalled forks and arrests division, thereby preserving genome integrity and promoting cell survival. In budding yeast, Tof1 is thought to act as a specific mediator of the replication checkpoint signal that activates the effector kinase Rad53. Here we report studies of fission yeast Swi1, a Tof1-related protein required for a programmed fork-pausing event necessary for mating type switching. Our studies have shown that Swi1 is vital for proficient activation of the Rad53-like checkpoint kinase Cds1. Together they are required to prevent fork collapse in the ribosomal DNA repeats, and they also prevent irreversible fork arrest at a newly identified hydroxyurea pause site. Swi1 also has Cds1-independent functions. Rad22 DNA repair foci form during S phase in
swi1
mutants and to a lesser extent in
cds1
mutants, indicative of fork collapse. Mus81, a DNA endonuclease required for recovery from collapsed forks, is vital in
swi1
but not
cds1
mutants. Swi1 is recruited to chromatin during S phase. We propose that Swi1 stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors.
Metrics
Details
- Title
- Swi1 Prevents Replication Fork Collapse and Controls Checkpoint Kinase Cds1
- Creators
- Eishi Noguchi - Department of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037Chiaki Noguchi - Department of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037Li-Lin Du - Department of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037Paul Russell - Department of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037
- Publication Details
- Molecular and cellular biology, v 23(21), pp 7861-7874
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000186055400037
- Scopus ID
- 2-s2.0-0142027841
- Other Identifier
- 991014877822804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology