Journal article
Swi1 and Swi3 Are Components of a Replication Fork Protection Complex in Fission Yeast
Molecular and cellular biology, v 24(19), pp 8342-8355
Oct 2004
PMID: 15367656
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Swi1 is required for programmed pausing of replication forks near the
mat1
locus in the fission yeast
Schizosaccharomyces pombe
. This fork pausing is required to initiate a recombination event that switches mating type. Swi1 is also needed for the replication checkpoint that arrests division in response to fork arrest. How Swi1 accomplishes these tasks is unknown. Here we report that Swi1 copurifies with a 181-amino-acid protein encoded by
swi3
+
. The Swi1-Swi3 complex is required for survival of fork arrest and for activation of the replication checkpoint kinase Cds1. Association of Swi1 and Swi3 with chromatin during DNA replication correlated with movement of the replication fork. s
wi1Δ
and
swi3Δ
mutants accumulated Rad22 (Rad52 homolog) DNA repair foci during replication. These foci correlated with the Rad22-dependent appearance of Holliday junction (HJ)-like structures in cells lacking Mus81-Eme1 HJ resolvase. Rhp51 and Rhp54 homologous recombination proteins were not required for viability in
swi1Δ
or
swi3Δ
cells, indicating that the HJ-like structures arise from single-strand DNA gaps or rearranged forks instead of broken forks. We propose that Swi1 and Swi3 define a fork protection complex that coordinates leading- and lagging-strand synthesis and stabilizes stalled replication forks.
Metrics
Details
- Title
- Swi1 and Swi3 Are Components of a Replication Fork Protection Complex in Fission Yeast
- Creators
- Eishi Noguchi - Departments of Molecular BiologyChiaki Noguchi - Departments of Molecular BiologyW. Hayes McDonald - Departments of Molecular BiologyJohn R Yates - Departments of Molecular BiologyPaul Russell - Departments of Molecular Biology
- Publication Details
- Molecular and cellular biology, v 24(19), pp 8342-8355
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000223990100003
- Scopus ID
- 2-s2.0-4544250127
- Other Identifier
- 991014878050404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology