Journal article
Synergistic activation of retinal capillary pericyte proliferation in culture by inositol triphosphate and diacylglycerol
Experimental eye research, v 44(1)
1987
PMID: 3104074
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Inositol triphosphate (IP
3) and diacylglycerol (DG) are second messengers which control ionic events implicated in cell proliferation in a variety of tissues. In order to determine if these two second messengers control the proliferation of bovine retinal capillary pericytes (BRCP) or feline retinal pigment epithelial cells (FRPE) in culture, both intact BRCP or FRPE or BRCP or FRPE made permeable by saponin were used to study the effects of IP
3 and DG on [
3H]thymidine incorporation into DNA. [
3H]Thymidine incorporation by BRCP made permeable to saponin showed specific IP
3 dose-dependence; the apparent
K
m was 0·3 μM of IP
3. Similar effects of A23187, a Ca
2+ ionophore, or synthetic DG (1-oleoyl-2 acetyl-glycerol) were also observed. The combination of synthetic DG (0-, 2-, 4-, 8 μg ml
−1) and 1 μM A23187 produced greater stimulation of [
3H]thymidine incorporation by intact BRCP than was seen with DG or A23187 alone. In contrast to BRCP. [
3H]thymidine incorporation by FRPE was not stimulated by IP
3, A23187 or synthetic DG. The synergistic activation of IP
3 and DG provided direct evidence to support the view that BRCP proliferation in vitro were regulated by the levels of the two second messengers.
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Details
- Title
- Synergistic activation of retinal capillary pericyte proliferation in culture by inositol triphosphate and diacylglycerol
- Creators
- Weiye Li - University of PennsylvaniaTian Sheng Hu - University of PennsylvaniaLawrence E. Stramm - University of PennsylvaniaJohn H. Rockey - Peking Union Medical College HospitalShi Lian Liu - Peking Union Medical College Hospital
- Publication Details
- Experimental eye research, v 44(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Ophthalmology [Historical]
- Web of Science ID
- WOS:A1987G056600003
- Scopus ID
- 2-s2.0-0023139211
- Other Identifier
- 991019298823104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Ophthalmology