Journal article
Synergy between CD28 and CD9 costimulation for naive T-cell activation
Immunology letters, v 58(1)
1997
PMID: 9436464
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Our previous study demonstrated that CD9 is expressed on most mature naive T-cells and delivers a potent costimulatory signal that functions independently of CD28. Here, we investigated whether this CD9-mediated signal is different from the CD28-mediated signal in the mode of costimulation and whether both signals function synergistically for T-cell activation. Anti-CD9 or anti-CD28 monoclonal antibody (mAb) increased [
3H]TdR incorporation of naive T-cells in the absence of antigen-presenting cells (APC) when coimmobilized with submitogenic doses of anti-CD3 mAb. The levels of costimulation induced by ligation of CD9 and CD28 were comparable. However, the costimulatory effect differed between soluble anti-CD9 and CD28 mAb. A soluble form of anti-CD28 mAb could costimulate anti-CD3-triggered T-cells, whereas soluble anti-CD9 mAb failed to costimulate. Although anti-CD28 costimulated naive T-cells treated with phorbol myristate acetate (PMA) instead of anti-CD3 mAb, a combination of PMA plus anti-CD9 mAb could not induce T-cell activation. The combined costimulation of anti-CD3-triggered T-cells with anti-CD9 and anti-CD28 mAbs resulted in strikingly enhanced [
3H]TdR uptake and lymphokine (IL-2 and IFN-
γ) production when compared to those induced by each costimulation. These results suggest that CD9 and CD28 induce T-cell costimulation using different signaling pathways, thereby inducing synergy in T-cell activation.
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Details
- Title
- Synergy between CD28 and CD9 costimulation for naive T-cell activation
- Creators
- Kazuhito Toyo-oka - The University of OsakaXu-Guang Tai - The University of OsakaYumi Yashiro - The University of OsakaHyun-Jong Ahn - The University of OsakaRyo Abe - The University of TokyoToshiyuki Hamaoka - The University of OsakaMichiko Kobayashi - Genetics Institute, Inc.Steven Neben - Genetics Institute, Inc.Hiromi Fujiwara - The University of Osaka
- Publication Details
- Immunology letters, v 58(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1997XE10300004
- Scopus ID
- 2-s2.0-0030968784
- Other Identifier
- 991020099654404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology