Journal article
Synthesis, Structure Characterization and Antitumor Activity Study of a New Iron(III) Complex of 5-Nitro-8-hydroxylquinoline (HNOQ)
Chemical & pharmaceutical bulletin, v 64(8), pp 1208-1217
01 Aug 2016
PMID: 27238362
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A new iron(III) complex (1) of 5-nitro-8-hydroxylquinoline (HNOQ) was synthesized and structurally characterized in its solid state and solution state by IR, UV-Vis, electrospray ionization (ESI)-MS, elemental analysis, conductivity and X-ray single crystal diffraction analysis. The DNA binding study suggested that complex 1 interacted with calf thymus (ct)-DNA mainly via an intercalative binding mode. By 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay, the in vitro cytotoxicity of complex 1, comparing with HNOQ and cisplatin, was screened towards a series of tumor cell lines as well as the normal liver cell line HL-7702. Complex 1 showed higher cytotoxicity towards the tested tumor cell lines but lower cytotoxicity towards HL-7702 than HNOQ, in which the T-24 was the most sensitive cell line for 1. Complex 1 caused G2 phase cell cycle arrest and induced cell apoptosis in T-24 cells in a dose-dependent mode, evidenced by changes in cell morphology. Targeting the mitochondrial pathway due to the redox potential of Fe(III)/Fe(II), the apoptotic mechanism in T-24 cells treated by 1 was investigated by reactive oxygen species (ROS) detection, intracellular [Ca2+] measurement and caspase-9 and caspase-3 activity assay. It suggested that complex 1 induced cell apoptosis by triggering the caspase-9 and caspase-3 activation via a mitochondrion -mediated pathway.
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Details
- Title
- Synthesis, Structure Characterization and Antitumor Activity Study of a New Iron(III) Complex of 5-Nitro-8-hydroxylquinoline (HNOQ)
- Creators
- Hai-Rong Zhang - Central South UniversityTing Meng - Guangxi Normal UniversityYan-Cheng Liu - Guangxi Normal UniversityQi-Pin Qin - Guangxi Normal UniversityZhen-Feng Chen - Guangxi Normal UniversityYou-Nian Liu - Central South UniversityHong Liang - Central South UniversityaCollege of Chemistry and Chemical Engineering
- Publication Details
- Chemical & pharmaceutical bulletin, v 64(8), pp 1208-1217
- Publisher
- Pharmaceutical Soc Japan
- Number of pages
- 10
- Grant note
- 21271051; 21561005 / National Natural Science Foundations of China; National Natural Science Foundation of China (NSFC) Doctoral Construction Program CMEMR2013-C05; CMEMR2012-B01/2015-A10 / State Key Laboratory Foundation of Guangxi Normal University
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000381234500019
- Scopus ID
- 2-s2.0-84982678292
- Other Identifier
- 991019320614404721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Medicinal
- Chemistry, Multidisciplinary
- Pharmacology & Pharmacy