Journal article
Synthesis and characterization of a biotin-alginate conjugate and its application in a biosensor construction
Biomacromolecules, v 5(2), pp 389-396
Mar 2004
PMID: 15002998
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Biotin was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a biotin-alginate conjugate for subsequent use in biosensor applications. The synthetic procedure was optimized with respect to pH of the reaction medium (pH 6.0), the degree of uronic acid activation (20%), and the order of addition of the reagents. The biotin-alginate conjugate was characterized by titration with 2-anilinonaphthalene-6-sulfonic acid (2,6-ANS), 4-hydroxyazobene-2'-carboxylic acid (HABA) and by an HPSEC-MALLS analytical method as well as by FTIR and 13C NMR spectroscopy. As a compromise between the need for a high percent of molar modification of the alginate, on one hand, and sufficient gelling capability, on the other hand, an optimal modification of 10-13% of biotin-alginate was used. The new biotin-alginate conjugate was used for the encapsulation of bioluminescent reporter cells into microspheres. A biosensor was prepared by conjugating these biotinylated alginate microspheres to the surface of a streptavidin-coated optical fiber, and the performance of the biosensor was demonstrated in the determination of the antibiotic, mitomycin C as a model toxin.
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Details
- Title
- Synthesis and characterization of a biotin-alginate conjugate and its application in a biosensor construction
- Creators
- Boris Polyak - The Institute for Applied Biosciences and Department of Biotechnology Engineering, Ben-Gurion University of the Negev, PO Box 653, Beer Sheva 84105, IsraelShimona GereshRobert S Marks
- Publication Details
- Biomacromolecules, v 5(2), pp 389-396
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Surgery
- Web of Science ID
- WOS:000220109200018
- Scopus ID
- 2-s2.0-1642525682
- Other Identifier
- 991014877976504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Organic
- Polymer Science