Journal article
TCP4-dependent induction of CONSTANS transcription requires GIGANTEA in photoperiodic flowering in Arabidopsis
PLoS genetics, v 13(6), pp e1006856-e1006856
19 Jun 2017
PMID: 28628608
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Photoperiod is one of the most reliable environmental cues for plants to regulate flowering timing. In Arabidopsis thaliana, CONSTANS (CO) transcription factor plays a central role in regulating photoperiodic flowering. In contrast to posttranslational regulation of CO protein, still little was known about CO transcriptional regulation. Here we show that the CINCINNATA (CIN) clade of class II TEOSINTE BRANCHED 1/ CYCLOIDEA/ PROLIFERATING CELL NUCLEAR ANTIGEN FACTOR (TCP) proteins act as CO activators. Our yeast one-hybrid analysis revealed that class II CIN-TCPs, including TCP4, bind to the CO promoter. TCP4 induces CO expression around dusk by directly associating with the CO promoter in vivo. In addition, TCP4 binds to another flowering regulator, GIGANTEA (GI), in the nucleus, and induces CO expression in a GI-dependent manner. The physical association of TCP4 with the CO promoter was reduced in the gi mutant, suggesting that GI may enhance the DNA-binding ability of TCP4. Our tandem affinity purification coupled with mass spectrometry (TAP-MS) analysis identified all class II CIN-TCPs as the components of the in vivo TCP4 complex, and the gi mutant did not alter the composition of the TCP4 complex. Taken together, our results demonstrate a novel function of CIN-TCPs as photoperiodic flowering regulators, which may contribute to coordinating plant development with flowering regulation.
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Details
- Title
- TCP4-dependent induction of CONSTANS transcription requires GIGANTEA in photoperiodic flowering in Arabidopsis
- Creators
- Akane Kubota - University of WashingtonShogo Ito - University of WashingtonJae Sung Shim - University of WashingtonRichard S Johnson - University of WashingtonYong Hun Song - Ajou UniversityGhislain Breton - University of California San DiegoGreg S Goralogia - University of WashingtonMichael S Kwon - University of WashingtonDianne Laboy Cintrón - University of WashingtonTomotsugu Koyama - Suntory Foundation for Life SciencesMasaru Ohme-Takagi - Saitama UniversityJose L Pruneda-Paz - University of California San DiegoSteve A Kay - University of Southern CaliforniaMichael J MacCoss - University of WashingtonTakato Imaizumi - University of Washington
- Publication Details
- PLoS genetics, v 13(6), pp e1006856-e1006856
- Publisher
- Public LIbrary of Science (PLOS)
- Grant note
- P41 GM103533 / NIGMS NIH HHS R01 GM079712 / NIGMS NIH HHS RC2 GM092412 / NIGMS NIH HHS R01 GM067837 / NIGMS NIH HHS R25 HG007153 / NHGRI NIH HHS R37 GM067837 / NIGMS NIH HHS P30 AG013280 / NIA NIH HHS R01 GM056006 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000404512600033
- Scopus ID
- 2-s2.0-85021794031
- Other Identifier
- 991021838161004721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Genetics & Heredity