Journal article
TGF-beta induced epithelial-mesenchymal transition in an advanced cervical tumor model by 3D printing
Biofabrication, v 10(4), pp 044102-044102
01 Oct 2018
PMID: 30129928
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
An advanced in vitro cervical tumor model was established by 3D printing to study the epithelial-to-mesenchymal transition (EMT), which is a very important stage of dissemination of carcinoma leading to metastatic tumors. A HeLa/hydrogel grid construct composed of gelatin, alginate, Matrigel and HeLa cells was fabricated by forced extrusion in a layer-by-layer fashion. HeLa cells rapidly proliferated, formed spheroids and presented tumorigenic characteristic in the 3D-printed structure. With the supplement of TGF-beta, aggregated HeLa cells started to disintegrate, and some of them changed into fibroblast-like spindle morphology, which indicated that EMT was induced. The downregulation of epithelial marker E-cadherin, and up-regulation of mesenchymal markers such as snail, vimentin and N-cadherin were all observed in the 3D-printed model, and performed differently in 3D and 2D models. The TGF-beta induced EMT was inhibited by the treatment of disulfiram and EMT pathway inhibitor C19 in a dose dependent manner, showing great potential for future studies of a therapeutic program towards cervical tumor metastasis.
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Details
- Title
- TGF-beta induced epithelial-mesenchymal transition in an advanced cervical tumor model by 3D printing
- Creators
- Y. Pang - Tsinghua UniversityS. S. Mao - Tsinghua UniversityR. Yao - Tsinghua UniversityJ. Y. He - Tsinghua UniversityZ. Z. Zhou - Tsinghua UniversityL. Feng - State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People’s Republic of ChinaK. T. Zhang - State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People’s Republic of ChinaS. J. Cheng - State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, People’s Republic of ChinaW. Sun - Tsinghua University
- Publication Details
- Biofabrication, v 10(4), pp 044102-044102
- Publisher
- Iop Publishing Ltd
- Number of pages
- 12
- Grant note
- G2017002 / 111 Project; Ministry of Education, China - 111 Project 51235006 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 3184049 / Beijing Natural Science Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics; General Internal Medicine
- Web of Science ID
- WOS:000444433700001
- Scopus ID
- 2-s2.0-85055795747
- Other Identifier
- 991019167709704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials