Journal article
TGF-β isoforms in alcoholic liver disease
Journal of gastroenterology, v 33(3), pp 383-389
May 1998
PMID: 9658318
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The increased deposition of extracellular matrix proteins in the liver is a key factor in the morbidity and mortality of alcoholic liver disease (ALD). This increased fibrosis may be due to a superabundance of profibrogenic factors such as transforming growth factor-β (TGF-β). The original peptide is now called TGF-β1, and two other isoforms have been recognized in humans (TGF-β2 and TGF-β3). It was the aim of the present study to determine the expression of the TGF-β isoforms in different stages of ALD. Thirty patients with ALD had percutaneous liver biopsies performed for diagnostic purposes. They were grouped by clinical findings and by liver histology into four groups: I, steatosis; II, fibrosis; III, hepatitis; and IV, cirrhosis. An unused portion of each biopsy sample was used to evaluate the gene expression of TGF-β1, TGF-β2, and TGF-β3 by reverse transcription polymerase chain reaction (RT-PCR). The expression of all isoforms from patients was significantly greater than their expression in controls. No significant correlation was determined between TGF-β isoform expression and liver function test results. When the different isoforms were grouped by histology, increased expression with more severe disease was found; however, differences existed among the isoforms. In ALD, all TGF-β isoforms were increased and their expression was significantly greater in patients with more active and advanced disease. RT-PCR is an effective method for evaluating gene expression in clinical samples which often provide a limited amount of tissue.
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Details
- Title
- TGF-β isoforms in alcoholic liver disease
- Creators
- Rui M Santos - Medicina III, Hospitais da Universidade de Coimbra, Coimbra, Portugal PTPamela Norton - Department of Medicine, Jefferson Medical College, 1025 Walnut Street, Room 901, Philadelphia, PA, USA USSilvia Degli Esposti - Women's and Infants' Hospital, Brown University, Providence, RI, USA USMark A Zern - Department of Medicine, Jefferson Medical College, 1025 Walnut Street, Room 901, Philadelphia, PA, USA US
- Publication Details
- Journal of gastroenterology, v 33(3), pp 383-389
- Publisher
- Springer-Verlag; Tokyo
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000073766100013
- Scopus ID
- 2-s2.0-0031777399
- Other Identifier
- 991014878085004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Gastroenterology & Hepatology