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TLR and ODN agonists used as adjuvants enhance the protection elicited in mice by a vaccine formulated with the Chlamydia trachomatis MoPn rMOMP (106.17)
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TLR and ODN agonists used as adjuvants enhance the protection elicited in mice by a vaccine formulated with the Chlamydia trachomatis MoPn rMOMP (106.17)

Chunmei Chang, Pooja Jain, Sukumar Pal, Delia Tifrea and Luis de la Maza
The Journal of immunology (1950), v 186(1_Supplement), pp 106-106.17
01 Apr 2011
DOI: https://doi.org/10.4049/jimmunol.186.Supp.106.17

Abstract

Abstract Chlamydia trachomatis (CT) is the most common sexually transmitted bacterial pathogen in the World and there is an urgent need for a vaccine to prevent these infections. To determine what type of adjuvant better enhances the immunogenicity of a vaccine, we formulated the CT recombinant major outer membrane protein (rMOMP) with several TLR and NOD agonists including: Pam2CSK4 (TLR2/6), Poly(I:C) (TLR3), Monophosphoryl lipid A (TLR4), Flagellin (TLR5), Imiquimod R837 (TLR7), Imidazoquinoline R848 (TRL7/8), CpG-1826 (TLR9), M-Tri-DAP (NOD1/NOD2) and muramyldipeptide (NOD2). Groups of female BALB/c mice were immunized i.m. with rMOMP and one of those adjuvants and were challenged i.n. with 104 CT MoPn inclusion forming units (IFU). Following the challenge the mice were weighed and, at 10 days post-infection (p.i.), they were euthanized, their lungs weighed and the number of IFU in the lungs determined. Based on the IgG2a/IgG1 ratio in sera collected the day before the challenge, mice immunized with rMOMP-Pam2CSK4 showed a strong Th2 bias. Furthermore, these mice develop a robust cellular immune response with high CT-specific T cell proliferation and levels of IFN-γ. In addition, based on changes in body weight, weight of the lungs and number of IFU recovered from the lungs, mice immunized with rMOMP-Pam2CSK4, were significantly better protected than mice immunized with the other adjuvants. In summary, Pam2CSK should be considered as a good candidate adjuvant for a CT vaccine.

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