Journal article
TNF-α enhances Toxoplasma gondii cyst formation in human fibroblasts through the sphingomyelinase pathway
Cellular signalling, v 8(6), pp 439-442
01 Sep 1996
PMID: 8958446
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The cystogenesis event of
Toxoplasma gondii is poorly understood. In order to throw light on it, the effect of tumor necrosis factor-α (TNF-α) was studied in the Prugniaud strain of the organism. This showed that TNF-α increased the number of cysts formed
in vitro in human MRC5 fibroblasts. The sphingomyelinase pathway may be involved in mediating the TNF effect, since ceramide (natural form in permeabilized cells or cell-permeable analogue) could mimic the action of TNF. More precisely, our results strongly suggest the involvement of an acidic sphingomyelinase in mediating the effect of TNF; indeed, D609 inhibited both the TNF effect and cyst formation, while arachidonic acid had no effect. Moreover, protein kinase C (PKC) seems also to play a role in the process, since phorbol-12-myristate-13-acetate (PMA) enhanced the cyst formation. However, chelerythrine chloride did not prevent the TNF effect, suggesting that several host-cell pathways can affect the cystogenesis event. Taken together, these results suggest the active participation of host-cell components in the cystogenesis of
Toxoplasma gondii.
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Details
- Title
- TNF-α enhances Toxoplasma gondii cyst formation in human fibroblasts through the sphingomyelinase pathway
- Creators
- Jérôme Ricard - Merci (Czechia)Hervé Pelloux - Département de Chimie MoléculaireSoham Pathak - Département de Chimie MoléculaireBernard Pipy - Université Toulouse III - Paul SabatierPierre Ambroise-Thomas - Département de Chimie Moléculaire
- Publication Details
- Cellular signalling, v 8(6), pp 439-442
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:A1996VT60100005
- Scopus ID
- 2-s2.0-0042399599
- Other Identifier
- 991021229884704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology