Journal article
TPX2 regulates neuronal morphology through kinesin-5 interaction
Cytoskeleton (Hoboken, N.J.), v 72(7), pp 340-348
Jul 2015
PMID: 26257190
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
TPX2 (targeting protein for Xklp2) is a multifunctional mitotic spindle assembly factor that in mammalian cells localizes and regulates mitotic motor protein kinesin-5 (also called Eg5 or kif11). We previously showed that upon depletion or inhibition of kinesin-5 in cultured neurons, microtubule movements increase, resulting in faster growing axons and thinner dendrites. Here, we show that depletion of TPX2 from cultured neurons speeds their rate of process outgrowth, similarly to kinesin-5 inhibition. The phenotype is rescued by TPX2 re-expression, but not if TPX2's kinesin-5-interacting domain is deleted. These results, together with studies showing a spike in TPX2 expression during dendritic differentiation, suggest that the levels and distribution of TPX2 are likely to be determinants of when and where kinesin-5 acts in neurons.
Metrics
Details
- Title
- TPX2 regulates neuronal morphology through kinesin-5 interaction
- Creators
- Olga I Kahn - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PennsylvaniaNgoc Ha - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PennsylvaniaMichelle A Baird - National High Magnetic Field Laboratory and Department of Biological Science, Florida State University, Tallahassee, FloridaMichael W Davidson - National High Magnetic Field Laboratory and Department of Biological Science, Florida State University, Tallahassee, FloridaPeter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania
- Publication Details
- Cytoskeleton (Hoboken, N.J.), v 72(7), pp 340-348
- Publisher
- Wiley; United States
- Grant note
- R56 NS028785 / NINDS NIH HHS F31 MH101974 / NIMH NIH HHS R01 NS028785 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000361309700002
- Scopus ID
- 2-s2.0-84941174863
- Other Identifier
- 991014878061504721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology