Journal article
Targeted binding of PEG-lipid modified polymer ultrasound contrast agents with tiered surface architecture
Biotechnology and bioengineering, v 106(3), pp 501-506
15 Jun 2010
PMID: 20091738
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In order for site-directed polymer ultrasound contrast agents (UCAs) to provide acoustic enhancement at disease sites to distinguish normal tissue from diseased tissue, the surface of these agents must be functionalized with mixtures of graftedpolymers. Here a combination of longer liganded polyethylene glycol (PEG)-lipids and shorter unliganded PEG-lipids were introduced into the oil phase of a modified solvent evaporation double emulsion method for preparing UCAs. UCAs with different lengths of both liganded and unliganded lipids were imaged under 7.5 MHz ultrasound. The B-mode image brightness of the mixed PEG-lipid UCAs was within 1 dB the brightness of the unliganded surface. After 15 min of continuous insonation, 70% of the contrast signal remained. The peptide
Arginine-Glycine-Aspartic Acid
(RGD) was added to the surface of these UCAs through a biotin-avidin linkage and binding was assessed under static and shear conditions. Binding was significant after 30 min of static incubation and the adherence of the UCA increased under shear flow from 3 UCA/cell (static) to 5 UCA/cell (shear).
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Details
- Title
- Targeted binding of PEG-lipid modified polymer ultrasound contrast agents with tiered surface architecture
- Creators
- Wynter J Duncanson - Department of Biomedical Engineering, Boston UniversityKelleny Oum - School of Biomedical Engineering, Science and Health Systems, Drexel UniversityJohn R Eisenbrey - School of Biomedical Engineering, Science and Health Systems, Drexel UniversityRobin O Cleveland - Department of Mechanical Engineering, Boston UniversityMargaret A Wheatley - School of Biomedical Engineering, Science and Health Systems, Drexel UniversityJoyce Y Wong - Department of Biomedical Engineering, Boston University
- Publication Details
- Biotechnology and bioengineering, v 106(3), pp 501-506
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000277817300017
- Scopus ID
- 2-s2.0-77953548564
- Other Identifier
- 991014878079904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology