Journal article
Targeted nanobubbles in low-frequency ultrasound-mediated gene transfection and growth inhibition of hepatocellular carcinoma cells
Tumor biology, v 37(9), pp 12113-12121
Sep 2016
PMID: 27216880
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The use of SonoVue combined with ultrasound exposure increases the transfection efficiency of short interfering RNA (siRNA). The objective of this study was to prepare targeted nanobubbles (TNB) conjugated with NET-1 siRNA and an antibody GPC3 to direct nanobubbles to hepatocellular carcinoma cells. SMMC-7721 human hepatocellular carcinoma cells were treated with six different groups. The transfection efficiency and cellular apoptosis were measured by flow cytometry. The protein and messenger RNA (mRNA) expression were measured by Western blot and quantitative real-time PCR, respectively. The migration and invasion potential of the cells were determined by Transwell analysis. The results show that US-guided siRNA-TNB transfection effectively enhanced gene silencing. In summary, siRNA-TNB may be an effective delivery vector to mediate highly effective RNA interference in tumor treatment.
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Details
- Title
- Targeted nanobubbles in low-frequency ultrasound-mediated gene transfection and growth inhibition of hepatocellular carcinoma cells
- Creators
- Bolin Wu - Third Affiliated Hospital of Harbin Medical UniversityQiang Qiao - Third Affiliated Hospital of Harbin Medical UniversityXue Han - Third Affiliated Hospital of Harbin Medical UniversityHui Jing - Third Affiliated Hospital of Harbin Medical UniversityHao Zhang - Harbin Institute of TechnologyHongjian Liang - Harbin Institute of TechnologyWen Cheng - Third Affiliated Hospital of Harbin Medical University
- Publication Details
- Tumor biology, v 37(9), pp 12113-12121
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000387075400054
- Scopus ID
- 2-s2.0-84969787279
- Other Identifier
- 991019320404304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Oncology