Journal article
Targeting Membrane HDM-2 by PNC-27 Induces Necrosis in Leukemia Cells But Not in Normal Hematopoietic Cells
Anticancer research, v 40(9), pp 4857-4867
01 Sep 2020
PMID: 32878773
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background/Aim: Anticancer peptide PNC-27 binds to HDM-2 protein on cancer cell membranes inducing the formation of cytotoxic transmembrane pores. Herein, we investigated HDM-2 membrane expression and the effect of PNC-27 treatment on human non-stein cell acute myelogenous leukemia cell lines: U937, acute monocytic leukemia; OCI-AML3, acute myelomonocytic leukemia and HL60, acute promyelocytic leukemia. Materials and Methods: We measured cell surface membrane expression of HDM-2 using flow cytometry. Cell viability was assessed using MTT assay while direct cytotoxicity was measured by lactate dehydrogenase (LDH) release and induction of apoptotic markers annexin V and caspase-3. Results: HDM-2 is expressed at high levels in membranes of U937, OCIAML3 and HL-60 cells. PNC-27 can bind to membrane HDM-2 to induce cell necrosis and LDH release within 4 h. Conclusion: Targeting membrane HDM-2 can be a potential strategy to treat leukemia. PNC-27 targeting membrane HDM-2 demonstrated significant anti-leukemia activity in a variety of leukemic cell lines.
Metrics
Details
- Title
- Targeting Membrane HDM-2 by PNC-27 Induces Necrosis in Leukemia Cells But Not in Normal Hematopoietic Cells
- Creators
- Anusha Thadi - Drexel UniversityLauren Lewis - Drexel UniversityEve Goldstein - Drexel UniversityAnshu Aggarwal - Drexel UniversityMarian Khalili - Drexel UniversityLindsay Steele - Drexel UniversityBoris Polyak - Drexel UniversityShabnam Seydafkan - SUNY Downstate Medical CenterMartin H. Bluth - Wayne State UniversityKristine A. Ward - University of PennsylvaniaMichael Styler - Fox Chase Cancer CenterPaul M. Campbell - Fox Chase Cancer CenterMatthew R. Pincus - SUNY Downstate Medical CenterWilbur B. Bowne - Thomas Jefferson University
- Publication Details
- Anticancer research, v 40(9), pp 4857-4867
- Publisher
- Int Inst Anticancer Research
- Number of pages
- 11
- Grant note
- American Medical Association Oncolyze Inc.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Surgery
- Web of Science ID
- WOS:000568989900005
- Scopus ID
- 2-s2.0-85090260898
- Other Identifier
- 991019169797704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Oncology