Files and links (1)
Published, Version of Record (VoR)Open Access (License Unspecified), Open
Journal article
Temperature‐sensitive mutation in yeast mitochondrial ribosome recycling factor (RRF)
Nucleic acids research, v 31(14), pp 4218-4226
15 Jul 2003
PMID: 12853640
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The yeast protein Rrf1p encoded by the FIL1 nuclear gene bears significant sequence similarity to Escherichia coli ribosome recycling factor (RRF). Here, we call FIL1 Ribosome Recycling Factor of yeast, RRF1. Its gene product, Rrf1p, was localized in mitochondria. Deletion of RRF1 leads to a respiratory incompetent phenotype and to instability of the mitochondrial genome (conversion to rho–/rho0 cytoplasmic petites). Yeast with intact mitochondria and with deleted genomic RRF1 that harbors a plasmid carrying RRF1 was prepared from spores of heterozygous diploid yeast. Such yeast with a mutated allele of RRF1, rrf1‐L209P, grew on a non‐fermentable carbon source at 30 but not at 36°C, where mitochondrial but not total protein synthesis was 90% inhibited. We propose that Rrf1p is essential for mitochondrial protein synthesis and acts as a RRF in mitochondria.
Metrics
Details
- Title
- Temperature‐sensitive mutation in yeast mitochondrial ribosome recycling factor (RRF)
- Creators
- Emeline Teyssier - Thomas Jefferson UniversityGo Hirokawa - Department of Biochemistry and Molecular Pharmacology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107‐5541, USAAnna Tretiakova - Department of Biochemistry, Drexel University, College of Medicine, Philadelphia, PA 19102, USABradford Jameson - Drexel UniversityAkira Kaji - Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA andHideko Kaji - Department of Biochemistry and Molecular Pharmacology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107‐5541, USA
- Publication Details
- Nucleic acids research, v 31(14), pp 4218-4226
- Publisher
- Oxford University Press
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000184106700047
- Scopus ID
- 2-s2.0-0242286001
- Other Identifier
- 991019169813204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology