Journal article
Temporal and parental-specific expression of imprinted genes in a newly derived Chinese human embryonic stem cell line and embryoid bodies
Human molecular genetics, v 15(1), pp 65-75
01 Jan 2006
PMID: 16319131
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Although the study of imprinted genes in human development is very important, little is known about their expression and regulation in the early differentiation of human tissues due to lack of an appropriate model. In this study, a Chinese human embryonic stem (hES) cell line, SHhES1, was derived and fully characterized. Expression profiles of human imprinted genes were determined by Affymetrix Oligo micro-array in undifferentiated SHhES1 cells and SHhES1-derived embryoid bodies (EBs) at day 3, 8, 13 and 18. Thirty-two known human imprinted genes were detected in undifferentiated ES cells. Significantly, differential expression was found in nine genes at different stages of EB formation. Expression profile changes were confirmed by quantitative real-time reverse transcriptase–polymerase chain reaction in SHhES1 cells as well as in another independently derived hES cell line, HUES-7. In addition, the monoallelic expressions of four imprinted genes were examined in three different passages of undifferentiated ES cells and EBs of both hES cell lines. The monoallelic expressions of imprinted genes, H19, PEG10, NDNL1 and KCNQ1 were maintained in both undifferentiated hES cells and derived EBs. More importantly, with the availability of maternal peripheral blood lymphocyte sample, we demonstrated that the maternal expression of KCNQ1 and the paternal expression of NDNL1 and PEG10 were maintained in SHhES1 cells. These data provide the first demonstration that the parental-specific expression of imprinted genes is stable in EBs after extensive differentiation, also indicating that in vitro fertilization protocol does not disrupt the parental monoallelic expression of the imprinted genes examined.
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Details
- Title
- Temporal and parental-specific expression of imprinted genes in a newly derived Chinese human embryonic stem cell line and embryoid bodies
- Creators
- Bo Wen Sun - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaA. Cong Yang - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaYun Feng - Ruijin HospitalYi Juan Sun - Ruijin HospitalYu fei Zhu - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaYi Zhang - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaHua Jiang - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaChun Liang Li - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaFu Rong Gao - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaZhi Hong Zhang - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaWei Cheng Wang - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaXiang Yin Kong - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaGang Jin - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaShi Jun Fu - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, ChinaYing Jin - Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences, 225 South Chongqing Road, Shanghai 200025, China
- Publication Details
- Human molecular genetics, v 15(1), pp 65-75
- Publisher
- Oxford University Press
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000234219000007
- Scopus ID
- 2-s2.0-29644440148
- Other Identifier
- 991020545229604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Genetics & Heredity