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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Temporal and spatial distribution of macrophage phenotype markers in the foreign body response to glutaraldehyde-crosslinked gelatin hydrogels
Journal of biomaterials science. Polymer ed, v 27(8), pp 721-742
23 May 2016
PMID: 26902292
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Currently, it is not well understood how changes in biomaterial properties affect the foreign body response (FBR) or macrophage behavior. Because failed attempts at biomaterial degradation by macrophages have been linked to frustrated phagocytosis, a defining feature of the FBR, we hypothesized that increased hydrogel crosslinking density (and decreased degradability) would exacerbate the FBR. Gelatin hydrogels were crosslinked with glutaraldehyde (0.05, 0.1, and 0.3%) and implanted subcutaneously in C57BL/6 mice over the course of 3 weeks. Interestingly, changes in hydrogel crosslinking did not affect the thickness of the fibrous capsule surrounding the hydrogels, expression of the pan-macrophage marker F480, expression of three macrophage phenotype markers (iNOS, Arg1, CD163), or expression of the myofibroblast marker aSMA, determined using semi-quantitative immunohistochemical analysis. With respect to temporal changes, the level of expression of the M1 marker (iNOS) remained relatively constant throughout the study, while the M2 markers Arg1 and CD163 increased over time. Expression of these M2 markers was highly correlated with fibrous capsule thickness. Differences in spatial distribution of staining also were noted, with the strongest staining for iNOS at the hydrogel surface and increasing expression of the myofibroblast marker aSMA toward the outer edge of the fibrous capsule. These results confirm previous reports that macrophages in the FBR exhibit characteristics of both M1 and M2 phenotypes. Understanding the effects (or lack of effects) of biomaterial properties on the FBR and macrophage phenotype may aid in the rational design of biomaterials to integrate with surrounding tissue.
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Details
- Title
- Temporal and spatial distribution of macrophage phenotype markers in the foreign body response to glutaraldehyde-crosslinked gelatin hydrogels
- Creators
- Tony Yu - Drexel UniversityWenbo Wang - Shanghai Jiao Tong UniversitySina Nassiri - Drexel UniversityThomas Kwan - Keele UniversityChau Dang - Drexel UniversityWei Liu - Shanghai Jiao Tong UniversityKara L. Spiller - Drexel University
- Publication Details
- Journal of biomaterials science. Polymer ed, v 27(8), pp 721-742
- Publisher
- Taylor & Francis
- Grant note
- 1012766 / Burroughs Wellcome Fund (10.13039/100000861) HL130037-01 / National Heart, Lung, and Blood Institute (10.13039/100000050)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000374584900002
- Scopus ID
- 2-s2.0-84961209709
- Other Identifier
- 991019168197504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials
- Polymer Science