Journal article
Temporal changes in the fecal bacterial community in Holstein dairy calves from birth through the transition to a solid diet
PLOS ONE, v 15(9), e0238882
08 Sep 2020
PMID: 32898158
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The development of a robust microbiome is critical to the health of dairy calves, but relatively little is known about the progression of the microbiome through the weaning transition. In this study, fecal samples were obtained from ten female Holstein calves at 6 timepoints between 2-13 weeks of age. Calves were fed acidified milk until weaning at 8 weeks old and had access to starter grain throughout the study. Fecal samples were extracted for genomic DNA, PCR-amplified for the V1-V2 region of the 16S rRNA bacterial gene, sequenced on the Illumina MiSeq platform, and analyzed using the QIIME2 pipeline. Bacterial richness, estimated by number of observed species, and bacterial diversity, estimated by Shannon diversity index, both differed significantly between timepoints and both increased over time (P<0.05), with the largest increases occurring during weaning. Weighted and unweighted UniFrac analysis showed significant differences (P<0.05) between bacterial communities across timepoints; betadisper analysis revealed that the microbiomes of individual calves became more similar with time. Throughout the study, Firmicutes was the dominant phylum, followed by Bacteroidetes. Thirteen bacterial genera were found to be significantly influenced by time, includingFaecalibacterium,Clostridium, unclassifiedS24-7,Collinsella,Sharpea, andTreponema. UnclassifiedRuminococcaceaewas the most prevalent genus at timepoints 1, 3, 5, and 6, but different amplicon sequence variants were detected at each timepoint suggesting the presence of different species ofRuminococcaceaeat different times.Bacteroideswas the most prevalent genus at timepoint 2, andPrevotellawas most prevalent at timepoint 4. Our results indicate that there is considerable variation in the calf microbiome pre-weaning, but the microbial community stabilizes and becomes similar to the adult microbiome at weaning. Further studies to describe the phylogeny and functionality of core microbiota through the weaning transition are needed to improve health and reduce diarrhea in the neonatal period.
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Details
- Title
- Temporal changes in the fecal bacterial community in Holstein dairy calves from birth through the transition to a solid diet
- Publication Details
- PLOS ONE, v 15(9), e0238882
- Publisher
- PUBLIC LIBRARY SCIENCE; SAN FRANCISCO
- Grant note
- Walmoore Holsteins, Inc. and Lindsay Stover allowed us to conduct the study using their facilities. This research was funded by the United States Department of Agriculture National Institute of Food and Agriculture (Grant #PENV570262; https://nifa.usda.gov/us-states-and-territories/pennsylvania).This grant was received by DP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Drexel University
- Web of Science ID
- WOS:000570962500041
- Scopus ID
- 2-s2.0-85090750484
- Other Identifier
- 991021860679104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Microbiology