Journal article
Temporal profiles of cytoskeletal protein loss following traumatic axonal injury in mice
Neurochemical research, v 32(12), pp 2006-2014
Dec 2007
PMID: 17401646
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To examine the time course and relative extent of proteolysis of neurofilament and tubulin proteins after traumatic axonal injury (TAI), anesthetized mice were subjected to optic nerve stretch injury. Immunohistochemistry confirmed neurofilament accumulation within axonal swellings at 4, 24, and 72 h postinjury (n = 4 injured and 2 sham per time point). Immunoblotting of optic nerve homogenates (n = 5 injured and 1 sham at 0.5, 4, 24 or 72 h) revealed calpain-mediated spectrin proteolytic fragments after injury. Protein levels for NF68 progressively decreased from 0.5 h to 24 h postinjury, while NF200 and alpha-tubulin levels decreased acutely (0.5-4 h), with a secondary decline at 72 h postinjury. These data demonstrate that diffusely distributed TAI is associated not only with a localized accumulation of neurofilament proteins, but also significant decreases in total cytoskeletal protein levels which may be mediated, in part, by calpains. Protection of the axonal cytoskeleton represents a potential therapeutic target for axonal damage associated with injury or neurodegenerative diseases.
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Details
- Title
- Temporal profiles of cytoskeletal protein loss following traumatic axonal injury in mice
- Creators
- Gulyeter Serbest - Department of Neurosurgery, University of Pennsylvania, Philadelphia, PA, USAMatthew F BurkhardtRobert SimanRamesh RaghupathiKathryn E Saatman
- Publication Details
- Neurochemical research, v 32(12), pp 2006-2014
- Publisher
- Springer Nature; United States
- Grant note
- P50-NS08803 / NINDS NIH HHS R01 NS048234-01A2 / NINDS NIH HHS R01-NS41561 / NINDS NIH HHS R01 NS048234 / NINDS NIH HHS R01-NS45131 / NINDS NIH HHS T32-NS043126 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000250626800002
- Scopus ID
- 2-s2.0-35848954542
- Other Identifier
- 991014877794304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences