Journal article
Textile-templated electrospun anisotropic scaffolds for regenerative cardiac tissue engineering
Biomaterials, v 35(30), pp 8540-8552
Oct 2014
PMID: 25017096
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
For patients with end-stage heart disease, the access to heart transplantation is limited due to the shortage of donor organs and to the potential for rejection of the donated organ. Therefore, current studies focus on bioengineering approaches for creating biomimetic cardiac patches that will assist in restoring cardiac function, by repairing and/or regenerating the intrinsically anisotropic myocardium. In this paper we present a simplified, straightforward approach for creating bioactive anisotropic cardiac patches, based on a combination of bioengineering and textile-manufacturing techniques in concert with nano-biotechnology based tissue-engineering stratagems. Using knitted conventional textiles, made of cotton or polyester yarns as template targets, we successfully electrospun anisotropic three-dimensional scaffolds from poly(lactic-co-glycolic) acid (PLGA), and thermoplastic polycarbonate-urethane (PCU, Bionate®). The surface topography and mechanical properties of textile-templated anisotropic scaffolds significantly differed from those of scaffolds electrospun from the same materials onto conventional 2-D flat-target electrospun scaffolds. Anisotropic textile-templated scaffolds electrospun from both PLGA and PCU, supported the adhesion and proliferation of H9C2 cardiac myoblasts cell line, and guided the cardiac tissue-like anisotropic organization of these cells in vitro. All cell-seeded PCU scaffolds exhibited mechanical properties comparable to those of a human heart, but only the cells on the polyester-templated scaffolds exhibited prolonged spontaneous synchronous contractility on the entire engineered construct for 10 days in vitro at a near physiologic frequency of ∼120 bpm. Taken together, the methods described here take advantage of straightforward established textile manufacturing strategies as an efficient and cost-effective approach to engineering 3D anisotropic, elastomeric PCU scaffolds that can serve as a cardiac patch.
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Details
- Title
- Textile-templated electrospun anisotropic scaffolds for regenerative cardiac tissue engineering
- Creators
- H. Gözde Şenel Ayaz - Drexel University, School of Biomedical Engineering, Science and Health Systems, Philadelphia, PA 19104, USAAnat Perets - Drexel University, School of Biomedical Engineering, Science and Health Systems, Philadelphia, PA 19104, USAHasan Ayaz - Drexel University, School of Biomedical Engineering, Science and Health Systems, Philadelphia, PA 19104, USAKyle D Gilroy - Temple University, Dept. Mechanical Engineering, College of Engineering, Philadelphia, PA 19122, USAMuthu Govindaraj - Philadelphia University, School of Engineering & Textiles, Philadelphia, PA 19144, USADavid Brookstein - Philadelphia University, School of Engineering & Textiles, Philadelphia, PA 19144, USAPeter I Lelkes - Temple University, Dept. Bioengineering, College of Engineering, Philadelphia, PA 19127, USA
- Publication Details
- Biomaterials, v 35(30), pp 8540-8552
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000340849600008
- Scopus ID
- 2-s2.0-84904737126
- Other Identifier
- 991014878537004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials