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Journal article
The 43-kDa neuronal growth-associated protein (GAP-43) is present in plasma membranes of rat astrocytes
Proceedings of the National Academy of Sciences - PNAS, v 85(21), pp 8296-8300
Nov 1988
PMID: 3054883
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Abstract
One of the neuronal growth-associated proteins, GAP-43 (molecular mass, approximately 43 kDa; pI 4.3), is abundant in growth-cone membranes and corresponds to a major protein kinase C substrate, the 46-kDa phosphoprotein (pp46), of a growth-cone-enriched subcellular fraction. This protein has the following additional designations (depending on context): B-50 (phospholipid metabolism), F1 (synaptic plasticity), and p57 (calmodulin binding). We show that a protein with the same molecular mass and isoelectric point as GAP-43, which interacts with anti-GAP-43 antibodies on immunoblots, is present in the plasma membranes of cultured neonatal rat cortical astrocytes. Double-immunofluorescence labeling of cells with a serum against glial fibrillary acidic protein and anti-GAP-43 antibody was observed. Furthermore, astrocytic protein was phosphorylated in vitro by protein kinase C and comigrated in two-dimensional PAGE with GAP-43. The data indicate that GAP-43, heretofore believed to be neuron-specific, is present in at least one class of glial cells.
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Details
- Title
- The 43-kDa neuronal growth-associated protein (GAP-43) is present in plasma membranes of rat astrocytes
- Creators
- L Vitković - National Institute of Neurological Disorders and StrokeH W Steisslinger - Laboratory of Molecular Biology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892V J Aloyo - Laboratory of Molecular Biology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892M Mersel - Laboratory of Molecular Biology, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 85(21), pp 8296-8300
- Publisher
- PNAS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1988Q834100099
- Scopus ID
- 2-s2.0-0023725913
- Other Identifier
- 991019183931704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Neurosciences