Journal article
The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication
Journal of virology, v 79(1), pp 225-233
Jan 2005
PMID: 15596818
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The human cytomegalovirus major immediate-early (α) protein IE1
491aa
plays an important role in controlling viral gene expression at low multiplicities of infection. With a transient complementation assay, full-length IE1
491aa
enhanced the growth of
ie
1 mutant virus CR208 20-fold better than a deletion mutant lacking 71 carboxyl-terminal amino acids (IE1
1-420aa
). A 16-amino-acid domain between amino acids 476 and 491 was both necessary and sufficient for chromatin-tethering activity; however, this domain was completely dispensable for complementation of CR208 replication. The proximal 55-amino-acid acidic domain (amino acids 421 to 475) was found to be most important for function. A deletion mutant lacking only this domain retained chromatin-tethering activity but failed to complement mutant virus. Interestingly, serine phosphorylation (at amino acids 399, 402, 406, 423, 428, 431, 448, 451, and 455) was not required for complementation. These results show that IE1
491aa
is composed of at least two domains that support replication, a region located between amino acids 1 and 399 that complements
ie
1 mutant virus replication to low levels and an acidic domain between amino acids 421 and 479 that dramatically enhances complementation.
Metrics
Details
- Title
- The Carboxyl-Terminal Region of Human Cytomegalovirus IE1491aa Contains an Acidic Domain That Plays a Regulatory Role and a Chromatin-Tethering Domain That Is Dispensable during Viral Replication
- Creators
- Jens Reinhardt - Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CaliforniaGeoffrey B Smith - Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CaliforniaChristopher T Himmelheber - Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CaliforniaJane Azizkhan-Clifford - Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CaliforniaEdward S Mocarski - Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, California
- Publication Details
- Journal of virology, v 79(1), pp 225-233
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000225904700022
- Scopus ID
- 2-s2.0-10644231699
- Other Identifier
- 991014878410904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Virology