Journal article
The Drosophila ortholog of the Zc3h14 RNA binding protein acts within neurons to pattern axon projection in the developing brain
Developmental neurobiology (Hoboken, N.J.), v 76(1)
Jan 2016
PMID: 25980665
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The dNab2 polyadenosine RNA binding protein is the D. melanogaster ortholog of the vertebrate ZC3H14 protein, which is lost in a form of inherited intellectual disability (ID). Human ZC3H14 can rescue D. melanogaster dNab2 mutant phenotypes when expressed in all neurons of the developing nervous system, suggesting that dNab2/ZC3H14 performs well-conserved roles in neurons. However, the cellular and molecular requirements for dNab2/ZC3H14 in the developing nervous system have not been defined in any organism. Here we show that dNab2 is autonomously required within neurons to pattern axon projection from Kenyon neurons into the mushroom bodies, which are required for associative olfactory learning and memory in insects. Mushroom body axons lacking dNab2 project aberrantly across the brain midline and also show evidence of defective branching. Coupled with the prior finding that ZC3H14 is highly expressed in rodent hippocampal neurons, this requirement for dNab2 in mushroom body neurons suggests that dNab2/ZC3H14 has a conserved role in supporting axon projection and branching. Consistent with this idea, loss of dNab2 impairs short-term memory in a courtship conditioning assay. Taken together these results reveal a cell-autonomous requirement for the dNab2 RNA binding protein in mushroom body development and provide a window into potential neurodevelopmental functions of the human ZC3H14 protein.
Metrics
Details
- Title
- The Drosophila ortholog of the Zc3h14 RNA binding protein acts within neurons to pattern axon projection in the developing brain
- Creators
- Seth M Kelly - Department of Biology, College of Wooster, Wooster, Ohio, 44691Rick Bienkowski - Graduate Program in Genetics and Molecular Biology, Emory University, Atlanta, Georgia, 30322Ayan Banerjee - Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, 30322David J Melicharek - Department of Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania, 19104Zachariah A Brewer - Department of Biology, College of Wooster, Wooster, Ohio, 44691Daniel R Marenda - Departments of Neurobiology & Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania, 19104Anita H Corbett - Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, 30322Kenneth H Moberg - Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia, 30322
- Publication Details
- Developmental neurobiology (Hoboken, N.J.), v 76(1)
- Publisher
- Wiley; United States
- Grant note
- R01 GM058728 / NIGMS NIH HHS T32 GM008367 / NIGMS NIH HHS MH107305 / NIMH NIH HHS T32 GM008490 / NIGMS NIH HHS 5K12 GM000680-12 / NIGMS NIH HHS GM058728 / NIGMS NIH HHS F31 HD079226 / NICHD NIH HHS K12 GM000680 / NIGMS NIH HHS R01 MH107305 / NIMH NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000366597500007
- Scopus ID
- 2-s2.0-84949729859
- Other Identifier
- 991014878544904721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Developmental Biology
- Neurosciences