Journal article
The Endogenous Mus81-Eme1 Complex Resolves Holliday Junctions by a Nick and Counternick Mechanism
Molecular cell, v 12(3), pp 747-759
01 Sep 2003
PMID: 14527419
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Functional studies strongly suggest that the Mus81-Eme1 complex resolves Holliday junctions (HJs) in fission yeast, but in vitro it preferentially cleaves flexible three-way branched structures that model replication forks or 3′ flaps. Here we report that a nicked HJ is the preferred substrate of endogenous and recombinant Mus81-Eme1. Cleavage occurs specifically on the strand that opposes the nick, resulting in resolution of the structure into linear duplex products. Resolving cuts made by the endogenous Mus81-Eme1 complex on an intact HJ are quasi-simultaneous, indicating that Mus81-Eme1 resolves HJs by a nick and counternick mechanism, with a large rate enhancement of the second cut arising from the flexible nature of the nicked HJ intermediate. Recombinant Mus81-Eme1 is ineffective at making the first cut. We also report that HJs accumulate in a DNA polymerase α mutant that lacks Mus81, providing further evidence that the Mus81-Eme1 complex targets HJs in vivo.
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Details
- Title
- The Endogenous Mus81-Eme1 Complex Resolves Holliday Junctions by a Nick and Counternick Mechanism
- Creators
- Pierre-Henri L Gaillard - Scripps Research InstituteEishi Noguchi - Scripps Research InstitutePaul Shanahan - Scripps Research InstitutePaul Russell - Scripps Research Institute
- Publication Details
- Molecular cell, v 12(3), pp 747-759
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000185613800022
- Scopus ID
- 2-s2.0-0141707818
- Other Identifier
- 991020834418104721
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology