Journal article
The Role of Apoptosis in Creating and Maintaining Luminal Space within Normal and Oncogene-Expressing Mammary Acini
Cell (Cambridge), v 111(1), pp 29-40
2002
PMID: 12372298
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have utilized in vitro three-dimensional epithelial cell cultures to analyze the role of apoptosis in the formation and maintenance of a hollow glandular architecture. Lumen formation is associated with the selective apoptosis of centrally located cells; this apoptosis follows apicobasal polarization and precedes proliferative suppression during acinar development. Notably, either inhibiting apoptosis (by exogenously expressing antiapoptotic Bcl family proteins) or enhancing proliferation (via Cyclin D1 or HPV E7 overexpression) does not result in luminal filling, suggesting glandular architecture is resistant to such isolated oncogenic insults. However, the lumen is filled when oncogenes that enhance proliferation are coexpressed with those that inhibit apoptosis, or when ErbB2, which induces both activities, is activated by homodimerization. Hence, apoptosis can counteract increased proliferation to maintain luminal space, suggesting that tumor cells must restrain apoptosis to populate the lumen.
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Details
- Title
- The Role of Apoptosis in Creating and Maintaining Luminal Space within Normal and Oncogene-Expressing Mammary Acini
- Creators
- Jayanta Debnath - Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USAKenna R Mills - Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USANicole L Collins - Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USAMauricio J Reginato - Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USASenthil K Muthuswamy - Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USAJoan S Brugge - Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USA
- Publication Details
- Cell (Cambridge), v 111(1), pp 29-40
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000178461900006
- Scopus ID
- 2-s2.0-0037020196
- Other Identifier
- 991014877662104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology