Journal article
The Tsk2/+ Mouse Fibrotic Phenotype Is Due to a Gain-of-Function Mutation in the PIIINP Segment of the Col3a1 Gene
Journal of investigative dermatology, v 135(3), pp 718-727
Mar 2015
PMID: 25330296
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Systemic sclerosis (SSc) is a polygenic, autoimmune disorder of unknown etiology, characterized by the excessive accumulation of extracellular matrix (ECM) proteins, vascular alterations, and autoantibodies. The tight skin (Tsk)2/+ mouse model of SSc demonstrates signs similar to SSc including tight skin and excessive deposition of dermal ECM proteins. By linkage analysis, we mapped the Tsk2 gene mutation to <3 megabases on chromosome 1. We performed both RNA sequencing of skin transcripts and genome capture DNA sequencing of the region spanning this interval in Tsk2/+ and wild-type littermates. A missense point mutation in the procollagen III amino terminal propeptide segment (PIIINP) of collagen, type III, alpha 1 (Col3a1) was found to be the best candidate for Tsk2; hence, both in vivo and in vitro genetic complementation tests were used to prove that this Col3a1 mutation is the Tsk2 gene. All previously documented mutations in the human Col3a1 gene are associated with the Ehlers–Danlos syndrome, a connective tissue disorder that leads to a defect in type III collagen synthesis. To our knowledge, the Tsk2 point mutation is the first documented gain-of-function mutation associated with Col3a1, which leads instead to fibrosis. This discovery provides insight into the mechanism of skin fibrosis manifested by Tsk2/+ mice.
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Details
- Title
- The Tsk2/+ Mouse Fibrotic Phenotype Is Due to a Gain-of-Function Mutation in the PIIINP Segment of the Col3a1 Gene
- Creators
- Kristen B. Long - Drexel UniversityZhenghui Li - Dartmouth CollegeChelsea M. Burgwin - Drexel UniversitySusanna G. Choe - Dartmouth CollegeViktor Martyanov - Dartmouth CollegeSihem Sassi-Gaha - Drexel UniversityJosh P. Earl - Center for Genomic Sciences, Pittsburgh, Pennsylvania, USARory A. Eutsey - Center for Genomic Sciences, Pittsburgh, Pennsylvania, USAAzad Ahmed - Genomic (Brazil)Garth D. Ehrlich - Genomic (Brazil)Carol M. Artlett - Drexel UniversityMichael L. Whitfield - Dartmouth CollegeElizabeth P. Blankenhorn - Drexel University
- Publication Details
- Journal of investigative dermatology, v 135(3), pp 718-727
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000349428600014
- Scopus ID
- 2-s2.0-84922636986
- Other Identifier
- 991019168655204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Dermatology