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Journal article
The analysis of clonal expansions in normal and autoimmune B cell repertoires
Philosophical transactions of the Royal Society of London. Series B. Biological sciences, v 370(1676), 20140239
05 Sep 2015
PMID: 26194753
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Clones are the fundamental building blocks of immune repertoires. The number of different clones relates to the diversity of the repertoire, whereas their size and sequence diversity are linked to selective pressures. Selective pressures act both between clones and within different sequence variants of a clone. Understanding how clonal selection shapes the immune repertoire is one of the most basic questions in all of immunology. But how are individual clones defined? Here we discuss different approaches for defining clones, starting with how antibodies are diversified during different stages of B cell development. Next, we discuss how clones are defined using different experimental methods. We focus on high-throughput sequencing datasets, and the computational challenges and opportunities that these data have for mining the antibody repertoire landscape. We discuss methods that visualize sequence variants within the same clone and allow us to consider collections of shared mutations to determine which sequences share a common ancestry. Finally, we comment on features of frequently encountered expanded B cell clones that may be of particular interest in the setting of autoimmunity and other chronic conditions.
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Details
- Title
- The analysis of clonal expansions in normal and autoimmune B cell repertoires
- Creators
- Uri Hershberg - Drexel UniversityEline T. Luning Prak (Corresponding Author) - Raymond and Ruth Perelman School of Medicine at the University of Pennsylvania
- Publication Details
- Philosophical transactions of the Royal Society of London. Series B. Biological sciences, v 370(1676), 20140239
- Publisher
- The Royal Society
- Number of pages
- 16
- Grant note
- Lupus Research Institute (ELP) P01AI106697 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) P01 AI106697 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000359514600002
- Scopus ID
- 2-s2.0-84937675261
- Other Identifier
- 991019167706304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biology