Journal article
The antifibrinolytic effects of carbon monoxide-releasing molecule-2 are fibrin and alpha(2)-antiplasmin dependent
Blood coagulation & fibrinolysis, v 21(6), pp 584-587
01 Sep 2010
PMID: 20683324
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Carbon monoxide derived from a carbon monoxide-releasing molecule [tricarbonyldichlororuthenium(II) dimer; CORM-2] has been recently demonstrated to diminish tissue-type plasminogen activator (tPA)-mediated fibrinolysis of plasma thrombi via enhancement of alpha(2)-antiplasmin-plasmin interactions. The goal of this study was to confirm this mechanism by comparing tPA-mediated fibrinolysis with fibrin-independent, alpha(2)-antiplasmin-resistant streptokinase-mediated fibrinolysis in CORM-2 exposed plasma. Normal plasma was exposed to 0 or 100 mu mol/l CORM-2, with coagulation activated with tissue factor and fibrinolysis initiated with 100 U/ml tPA or 50 U/ml streptokinase. Thrombus growth/disintegration kinetics were monitored with thrombelastography until clot lysis time occurred. Unlike tPA-lysed clots, streptokinase-exposed thrombi demonstrated no significant CORM-2-mediated prolongation of clot growth time or clot lysis time. In contrast, streptokinase-mediated lysis did not significantly change the CORM-2-mediated percentage increase in the maximum rate of clot growth or maximum rate of clot lysis compared with tPA-exposed thrombi. CORM-2 likely attenuates fibrinolysis by a fibrin-dependent/alpha(2)-antiplasmin-dependent mechanism. Additional molecular investigation (e. g., mass spectroscopy) is planned to further elucidate how CORM-2 modifies fibrinogen/fibrin. Blood Coagul Fibrinolysis 21:584-587 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
Metrics
Details
- Title
- The antifibrinolytic effects of carbon monoxide-releasing molecule-2 are fibrin and alpha(2)-antiplasmin dependent
- Creators
- Vance G. Nielsen - Drexel University
- Publication Details
- Blood coagulation & fibrinolysis, v 21(6), pp 584-587
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 4
- Grant note
- Department of Anesthesiology
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000281115200013
- Scopus ID
- 2-s2.0-77955918004
- Other Identifier
- 991019357765804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Hematology